Lezzi M, Gilbert L I
Proc Natl Acad Sci U S A. 1969 Oct;64(2):498-503. doi: 10.1073/pnas.64.2.498.
The injection of ecdysone (molting hormone) into chironomid larvae is known to result in the stimulation of specific gene activities (increased RNA synthesis). The morphological manifestation of this gene activation is the formation of a puff. Cytological and autoradiographic analysis of several regions of the salivary gland chromosomes of Chironomus tentans revealed that ecdysone also stimulates Balbiani ring 1. In contradistinction to the puffs, the Balbiani rings are tissue specific and account for a significant percentage of the cell's non-nucleolar RNA.The application of juvenile hormone results in a decreased activity of Balbiani ring 1, suggesting that the two hormones may act antagonistically. In addition, juvenile hormone induces a puff at chromosome region I-19-A and this puff becomes less active after a short-term treatment with ecdysone. These data demonstrate an effect of juvenile hormone on puffing and are the first to demonstrate activation of a Balbiani ring by ecdysone.
已知向摇蚊幼虫注射蜕皮激素（蜕皮激素）会刺激特定基因活性（增加RNA合成）。这种基因激活的形态学表现是形成一个胀泡。对细摇蚊唾液腺染色体几个区域的细胞学和放射自显影分析表明，蜕皮激素也会刺激巴尔比亚尼环1。与胀泡不同，巴尔比亚尼环具有组织特异性，并且占细胞非核仁RNA的很大比例。保幼激素的应用会导致巴尔比亚尼环1的活性降低，这表明这两种激素可能起拮抗作用。此外，保幼激素会在染色体区域I-19-A诱导一个胀泡，并且在用蜕皮激素进行短期处理后，这个胀泡的活性会降低。这些数据证明了保幼激素对胀泡形成的影响，并且首次证明了蜕皮激素对巴尔比亚尼环的激活作用。
