Anggorowati Nungki, Ratna Kurniasari Chatarina, Damayanti Karina, Cahyanti Titik, Widodo Irianiwati, Ghozali Ahmad, Romi Muhammad Mansyur, Sari Dwi Cahyani Ratna, Arfian Nur
Department of Anatomical Pathology, Medical Faculty, Sardjito Hospital, Universitas Gadjah Mada, Indonesia. Email:
Asian Pac J Cancer Prev. 2017 Mar 1;18(3):667-671. doi: 10.22034/APJCP.2017.18.3.667.
Background: Alpha-smooth muscle actin (α-SMA) is an isoform of actin, positive in myofibroblasts and is an epithelial to mesenchymal transition (EMT) marker. EMT is a process by which tumor cells develop to be more hostile and able to metastasize. Progression of tumor cells is always followed by cell composition and extracellular matrix component alteration. Increased α-SMA expression and collagen alteration may predict the progressivity of ovarian neoplasms. Objective: The aim of this research was to analyse the characteristic of α-SMA and collagen in tumor cells and stroma of ovarian neoplasms. In this study, PCNA (proliferating cell nuclear antigen) expression was also investigated. Methods: Thirty samples were collected including serous, mucinous, endometrioid, and clear cell subtypes. The expression of α-SMA and PCNA were calculated in cells and stroma of ovarian tumors. Collagen was detected using Sirius Red staining and presented as area fraction. Results: The overexpressions of α-SMA in tumor cells were only detected in serous and clear cell ovarian carcinoma. The histoscore of α-SMA was higher in malignant than in benign or borderline ovarian epithelial neoplasms (105.3±129.9 vs. 17.3±17.1, P=0.011; mean±SD). Oppositely, stromal α-SMA and collagen area fractions were higher in benign than in malignant tumors (27.2±6.6 vs 20.5±8.4, P=0.028; 31.0±5.6 vs. 23.7±6.4, P=0.04). The percentages of epithelial and stromal PCNA expressions were not significantly different between benign and malignant tumors. Conclusion: Tumor cells of serous and clear cell ovarian carcinoma exhibit mesenchymal characteristic as shown by α-SMA positive expression. This expression might indicate that these subtypes were more aggressive. This research showed that collagen and α-SMA area fractions in stroma were higher in benign than in malignant neoplasms.
α-平滑肌肌动蛋白（α-SMA）是肌动蛋白的一种同工型，在肌成纤维细胞中呈阳性，是上皮-间质转化（EMT）的标志物。EMT是肿瘤细胞变得更具侵袭性并能够转移的过程。肿瘤细胞的进展总是伴随着细胞组成和细胞外基质成分的改变。α-SMA表达增加和胶原蛋白改变可能预示卵巢肿瘤的进展性。目的：本研究旨在分析卵巢肿瘤的肿瘤细胞和基质中α-SMA和胶原蛋白的特征。本研究还调查了增殖细胞核抗原（PCNA）的表达。方法：收集30份样本，包括浆液性、黏液性、子宫内膜样和透明细胞亚型。计算卵巢肿瘤细胞和基质中α-SMA和PCNA的表达。使用天狼星红染色检测胶原蛋白，并以面积分数表示。结果：仅在浆液性和透明细胞卵巢癌的肿瘤细胞中检测到α-SMA的过表达。α-SMA的组织学评分在恶性卵巢上皮性肿瘤中高于良性或交界性肿瘤（105.3±129.9对17.3±17.1，P = 0.011；均值±标准差）。相反，良性肿瘤中基质α-SMA和胶原蛋白面积分数高于恶性肿瘤（27.2±6.6对20.5±8.4，P = 0.028；31.0±5.6对23.7±6.4，P = 0.04）。良性和恶性肿瘤之间上皮和基质PCNA表达的百分比无显著差异。结论：浆液性和透明细胞卵巢癌的肿瘤细胞表现出间充质特征，表现为α-SMA阳性表达。这种表达可能表明这些亚型更具侵袭性。本研究表明，良性肿瘤中基质中的胶原蛋白和α-SMA面积分数高于恶性肿瘤。
