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环磷酸鸟苷依赖性蛋白激酶使来自心脏和平滑肌的分离肌浆网中的受磷蛋白发生磷酸化。

Cyclic GMP-dependent protein kinase phosphorylates phospholamban in isolated sarcoplasmic reticulum from cardiac and smooth muscle.

作者信息

Raeymaekers L, Hofmann F, Casteels R

机构信息

Laboratory of Physiology, University of Leuven, Belgium.

出版信息

Biochem J. 1988 May 15;252(1):269-73. doi: 10.1042/bj2520269.

DOI:10.1042/bj2520269
PMID:2844148
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1149133/
Abstract

Phospholamban of isolated sarcoplasmic reticulum of cardiac and smooth muscle is phosphorylated by cyclic GMP-dependent protein kinase (G-kinase). Concomitantly, the affinity of the Ca2+ pump for Ca2+ is increased. These effects are very similar to those seen with cyclic AMP-dependent protein kinase (A-kinase). The phosphate incorporation into phospholamban and the stimulatory effects of both kinases on the Ca2+ pump are not additive, suggesting that G-kinase phosphorylates the same serine residue as A-kinase. A possible physiological role for phosphorylation of phospholamban by G-kinase is discussed.

摘要

心肌和平滑肌的分离肌浆网中的受磷蛋白可被环鸟苷酸依赖性蛋白激酶（G激酶）磷酸化。同时，钙泵对钙离子的亲和力增加。这些效应与环腺苷酸依赖性蛋白激酶（A激酶）所产生的效应非常相似。受磷蛋白中的磷酸盐掺入以及两种激酶对钙泵的刺激作用并非相加的，这表明G激酶与A激酶磷酸化的是同一个丝氨酸残基。文中讨论了G激酶使受磷蛋白磷酸化可能的生理作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec3d/1149133/d2a4d39faeb2/biochemj00231-0261-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec3d/1149133/d2a4d39faeb2/biochemj00231-0261-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec3d/1149133/d2a4d39faeb2/biochemj00231-0261-a.jpg

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Cyclic GMP-dependent protein kinase phosphorylates phospholamban in isolated sarcoplasmic reticulum from cardiac and smooth muscle.环磷酸鸟苷依赖性蛋白激酶使来自心脏和平滑肌的分离肌浆网中的受磷蛋白发生磷酸化。
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