Gagné Pierre-Luc, Fortier Marlène, Fraser Marc, Parent Lise, Vaillancourt Cathy, Verreault Jonathan
Centre de recherche en toxicologie de l'environnement (TOXEN), Département des sciences biologiques, Université du Québec à Montréal, C.P. 8888, succursale Centre-ville, Montreal, QC, H3C 3P8, Canada; Center for Interdisciplinary Research on Well-Being, Health, Society and Environment (CINBIOSE), Université du Québec à Montréal, C.P. 8888, Succursale Centre-ville, Montreal, QC, H3C 3P8, Canada.
INRS-Institut Armand-Frappier, Université du Québec, 531 boulevard des Prairies, Laval, QC, H7V 1B7, Canada.
Aquat Toxicol. 2017 Jul;188:26-32. doi: 10.1016/j.aquatox.2017.04.009. Epub 2017 Apr 17.
Dechlorane Plus (DP) is a chlorinated flame retardant used mainly in electrical wire and cable coating, computer connectors, and plastic roofing materials. Concentrations of DP (syn and anti isomers) are increasingly being reported in aquatic ecosystems worldwide. However, there is exceedingly little information on the exposure-related toxicity of DP in aquatic organisms, especially in bivalves. The objective of this study was to investigate the in vivo and in vitro effects of DP exposure on histopathology, lipid peroxidation (LPO) levels, cyclooxygenase (COX) activity, phagocytosis capacity and efficiency, and DNA strand breakage in the blue mussel (Mytilus edulis) following a 29days exposure (0.001, 0.01, 0.1 and 1.0μg DP/L). Blue mussels accumulated DP in muscle and digestive gland in a dose-dependent manner. LPO levels in gills were found to increase by 82% and 67% at the 0.01 and 1.0μg DP/L doses, respectively, while COX activity in gills decreased by 44% at the 1μg/L dose. No histopathological lesion was found in gonads following DP exposure. Moreover, no change in hemocyte DNA strand breakage, phagocytosis rate, and viability was observed following DP exposure. Present study showed that toxicity of DP may occur primarily via oxidative stress in the blue mussel and potentially other bivalves, and that gills represent the most responsive tissue to this exposure.
十氯联苯（DP）是一种氯化阻燃剂，主要用于电线电缆涂层、电脑连接器和塑料屋顶材料。全球水生生态系统中DP（顺式和反式异构体）的浓度报告越来越多。然而，关于DP对水生生物尤其是双壳贝类的暴露相关毒性的信息极其稀少。本研究的目的是调查在29天暴露（0.001、0.01、0.1和1.0μg DP/L）后，DP暴露对蓝贻贝（Mytilus edulis）的组织病理学、脂质过氧化（LPO）水平、环氧化酶（COX）活性、吞噬能力和效率以及DNA链断裂的体内和体外影响。蓝贻贝肌肉和消化腺中DP的积累呈剂量依赖性。鳃中LPO水平在0.01和1.0μg DP/L剂量下分别增加了82%和67%，而鳃中COX活性在1μg/L剂量下降低了44%。DP暴露后性腺未发现组织病理学损伤。此外，DP暴露后血细胞DNA链断裂、吞噬率和活力未观察到变化。本研究表明，DP的毒性可能主要通过蓝贻贝以及潜在的其他双壳贝类中的氧化应激发生，并且鳃是对这种暴露最敏感的组织。
