Na R, Stender I M, Wulf H C
Department of Dermatology, Bispebjerg Hospital, Copenhagen University, Denmark.
Acta Derm Venereol. 2001 Aug-Sep;81(4):246-9. doi: 10.1080/00015550152572859.
Fluorescence detection may help to demarcate skin cancer from normal skin, thus to reduce the potential of incomplete treatment resulting from unawareness of tumour extension in surrounding skin. In this study we evaluated the difference between autofluorescence of basal cell carcinomas (n = 21) and the normal-appearing skin surrounding them. Referring to the difference found, a point-by-point measurement was taken from the tumour lesions outwards to the surrounding skin to locate the differentiation point of autofluorescence on the skin. Protoporphyrin IX fluorescence was measured from the same spots using the same procedure, after the tumours and the surrounding skin had been treated with topical 5-aminolevulinic acid methyl ester cream. The point-by-point measurement enabled us to locate the vanishing point of the protoporphyrin IX peak, which was compared with the differentiation point of autofluorescence to assess the utility of autofluorescence in tumour demarcation. Illuminated by 370 nm light, both the tumour and surrounding skin emitted a fluorescence with peak intensity at 455+/-3 nm. The peak intensity was 53% (18-84%) (median, range) lower in the tumours than in normal skin (p<0.001). In 78% of the measurements, the differentiation point of the autofluorescence was within 3mm of the vanishing point of the protoporphyrin IX peak. Autofluorescence may be used in BCC demarcation.
荧光检测有助于区分皮肤癌与正常皮肤，从而降低因未察觉肿瘤在周围皮肤中的扩展而导致治疗不彻底的可能性。在本研究中，我们评估了21例基底细胞癌与其周围外观正常皮肤的自发荧光差异。根据所发现的差异，从肿瘤病变向外至周围皮肤进行逐点测量，以确定皮肤上自发荧光的分界点。在用局部5-氨基乙酰丙酸甲酯乳膏处理肿瘤及其周围皮肤后，使用相同程序从相同部位测量原卟啉IX荧光。逐点测量使我们能够确定原卟啉IX峰值的消失点，并将其与自发荧光的分界点进行比较，以评估自发荧光在肿瘤分界中的效用。在370nm光照射下，肿瘤和周围皮肤均发出峰值强度在455±3nm的荧光。肿瘤中的峰值强度比正常皮肤低53%（18-84%）（中位数，范围）（p<0.001）。在78%的测量中，自发荧光的分界点在原卟啉IX峰值消失点的3mm范围内。自发荧光可用于基底细胞癌的分界。
