Farahani M, Simic M G
Center for Radiation Research, National Bureau of Standards, Gaithersburg, Maryland 20899.
Biochemistry. 1988 Jun 28;27(13):4695-8. doi: 10.1021/bi00413a017.
Hydroxy radicals induce cross-linking between phenylalanine (Phe) and 2-deoxyribose (dR) via formation of corresponding free radical intermediates. The cross-linked products were separated and identified by capillary gas chromatography-mass spectrometry. When phenylalanine and 2-deoxyribose radicals were generated in a 1:1 ratio, the predominant interaction was between Phe and dR radicals while the Phe-Phe and dR-dR cross-links were less abundant. The newly discovered cross-link between 2-deoxyribose and phenylalanine may serve as a model for radiation or free radical induced cross-linking between DNA and proteins and in general between sugar moieties and amino acids.
羟基自由基通过形成相应的自由基中间体诱导苯丙氨酸（Phe）和2-脱氧核糖（dR）之间发生交联。交联产物通过毛细管气相色谱-质谱联用仪进行分离和鉴定。当苯丙氨酸和2-脱氧核糖自由基以1:1的比例生成时，主要的相互作用发生在Phe和dR自由基之间，而Phe-Phe和dR-dR交联则较少。新发现的2-脱氧核糖与苯丙氨酸之间的交联可能为辐射或自由基诱导的DNA与蛋白质之间以及一般糖基与氨基酸之间的交联提供一个模型。
