Ouenzar B, Weill D, Agoutin B, Keith G, Heyman T
Institut Curie, Centre Universitaire, Orsay, France.
Biochim Biophys Acta. 1988 Sep 7;950(3):429-34. doi: 10.1016/0167-4781(88)90140-6.
The relative amounts of iso-tRNAsGly and iso-tRNAsPro existing in chick embryo tendon are indicative of a specialization of the tRNA population for collagen synthesis. These amounts are not modified (i) in primary avian tendon (PAT) cells in culture for which the procollagen production varies from about 10% of total protein synthesis to 60% and (ii) in tendons from immature chicks, which show a 3-fold decrease of procollagen production with increasing age. The characteristic tRNA pattern was not maintained in cells which had lost the ability to make high levels of collagen as observed in the cases of: (i) PAT cells reaching confluency; (ii) virus-transformed PAT cells and (iii) tendon from adult chick. Our data are consistent with the idea that tendon tRNA specialization for collagen synthesis is a differentiation feature independent of the expression level of the collagenic function but related to its maintenance.
鸡胚肌腱中存在的异亮氨酸转运RNA（iso - tRNAsGly）和脯氨酸转运RNA（iso - tRNAsPro）的相对含量表明了用于胶原蛋白合成的转运RNA群体的特化。这些含量在以下情况中未发生改变：（i）培养中的原代禽肌腱（PAT）细胞，其前胶原产量从约占总蛋白质合成的10%变化到60%；（ii）来自未成熟雏鸡的肌腱，随着年龄增长，前胶原产量下降了3倍。在以下情况下观察到，失去产生高水平胶原蛋白能力的细胞中未维持特征性的转运RNA模式：（i）达到汇合状态的PAT细胞；（ii）病毒转化的PAT细胞；（iii）成年鸡的肌腱。我们的数据与以下观点一致，即肌腱转运RNA对胶原蛋白合成的特化是一种分化特征，与胶原功能的表达水平无关，但与其维持有关。
