Sharonov B P, Govorova N Iu, Lyzlova S N
Biokhimiia. 1988 May;53(5):816-25.
The ability of major serum proteins (albumin, immunoglobulin G) and free radical scavenger proteins (ceruloplasmin, superoxide dismutase, transferrin) to interact with O2-. and OCl- was studied. The interaction between serum proteins and OCl- was shown to be nonspecific and cause protein degradation. During SDS polyacrylamide gel electrophoresis ceruloplasmin and transferrin were degraded in the highest degree. Protein damage was also recorded by fluorescence changes. It is suggested that the damaging influence of active oxygen species secreted by stimulated neutrophils into the extracellular space can be abolished only by ceruloplasmin.
研究了主要血清蛋白（白蛋白、免疫球蛋白G）和自由基清除蛋白（铜蓝蛋白、超氧化物歧化酶、转铁蛋白）与超氧阴离子和次氯酸根离子相互作用的能力。血清蛋白与次氯酸根离子之间的相互作用是非特异性的，并导致蛋白质降解。在十二烷基硫酸钠聚丙烯酰胺凝胶电泳过程中，铜蓝蛋白和转铁蛋白的降解程度最高。蛋白质损伤也通过荧光变化记录下来。研究表明，只有铜蓝蛋白才能消除受刺激的中性粒细胞分泌到细胞外空间的活性氧的破坏作用。
