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用于区分均匀细胞悬液中死/活细菌的数据以及不溶性底物对比浊法测量的影响。

Data for discriminating dead/live bacteria in homogenous cell suspensions and the effect of insoluble substrates on turbidimetric measurements.

作者信息

Duedu Kwabena O, French Christopher E

机构信息

Institute of Quantitative Biology, Biochemistry and Biotechnology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3FF, UK.

Department of Biomedical Sciences, School of Basic & Biomedical Sciences, University of Health & Allied Sciences, Ho, Ghana.

出版信息

Data Brief. 2017 Apr 8;12:169-174. doi: 10.1016/j.dib.2017.04.003. eCollection 2017 Jun.

Abstract

Estimation of bacterial growth by rapid traditional methods such as spectrophometric measurements at 600 nm (OD600) is not applicable for cultures containing insoluble particles in the growth media. Colony counts are the only suitable alternative but these are laborious and not high-throughput. The data presented in this article is related to the research article entitled "Two-colour fluorescence fluorimetric analysis for direct quantification of bacteria and its application in monitoring bacterial growth in cellulose degradation systems" (Duedu and French, 2017) [1]. This data article presents original primary data describing the discrimination of dead/live bacteria in homogenous cell suspensions and how the presence of insoluble substrates affect the turbidity of the suspensions.

摘要

通过快速传统方法(如在600nm处进行分光光度测量(OD600))来估计细菌生长,不适用于生长培养基中含有不溶性颗粒的培养物。菌落计数是唯一合适的替代方法,但这些方法既费力又不是高通量的。本文所呈现的数据与题为《双色荧光荧光分析直接定量细菌及其在监测纤维素降解系统中细菌生长的应用》(杜埃杜和弗伦奇,2017年)[1]的研究文章相关。本数据文章呈现了原始的主要数据,描述了在均匀细胞悬浮液中区分死/活细菌的方法,以及不溶性底物的存在如何影响悬浮液的浊度。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20b1/5394208/d9953e280a7f/gr1.jpg

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