Velentzas Athanassios D, Velentzas Panagiotis D, Katarachia Stamatia, Mpakou Vassiliki E, Papassideri Issidora S, Stravopodis Dimitrios J
Section of Cell Biology and Biophysics, Department of Biology, School of Science, National and Kapodistrian University of Athens (NKUA), Athens, Greece.
Data Brief. 2017 Apr 8;12:180-183. doi: 10.1016/j.dib.2017.03.052. eCollection 2017 Jun.
This paper presents data associated with the research article entitled "Targeted downregulation of s36 protein unearths its cardinal role in chorion biogenesis and architecture during oogenesis" [1]. chorion is produced by epithelial follicle cells and one of its functional serving role is egg fertilization through the micropyle, a specialized narrow channel at the anterior tip of the egg [2]. Sperm entry during fertilization is necessary for the egg to complete meiosis [3]. flies being characterized by severe downregulation of the s36 chorionic protein, specifically in the follicle-cell compartment of their ovary, appear with impaired fly fertility (Velentzas et al., 2016) [1]. In an effort to further investigate whether the observed infertility in the s36-targeted flies derives from a fertilization failure, such as the inability of sperm to pass through egg׳s micropyle, we mated females carrying s36-depleted ovaries with males expressing the GFP protein either in their sperm tails, or in both their sperm tails and sperm heads.
本文展示了与题为《s36蛋白的靶向下调揭示其在卵子发生过程中绒毛膜生物发生和结构中的关键作用》的研究文章相关的数据[1]。绒毛膜由卵泡上皮细胞产生，其功能之一是通过卵孔实现卵子受精，卵孔是卵子前端一个特殊的狭窄通道[2]。受精过程中精子进入是卵子完成减数分裂所必需的[3]。果蝇的特征是s36绒毛膜蛋白严重下调，特别是在其卵巢的卵泡细胞区室，表现出果蝇生育力受损（Velentzas等人，2016年）[1]。为了进一步研究在靶向s36的果蝇中观察到的不育是否源于受精失败，例如精子无法穿过卵子的卵孔，我们将携带s36缺失卵巢的雌性果蝇与精子尾部或精子尾部和头部均表达绿色荧光蛋白（GFP）的雄性果蝇进行交配。
