Nagai Y, Yoshiba M
First Department of Medicine, Faculty of Medicine, University of Tokyo, Japan.
Clin Chim Acta. 1988 Jun 30;175(1):27-36. doi: 10.1016/0009-8981(88)90032-0.
The concentration of serum immunoreactive prolyl 4-hydroxylase (S-IRPH) was determined in patients with various liver diseases by the radioimmunoassay developed previously. S-IRPH values were elevated in acute hepatitis (p less than 0.01), hepatocellular carcinoma (p less than 0.05), metastatic liver neoplasm (p less than 0.01) and cholestatic diseases (p less than 0.001), but no significant elevation was seen in chronic hepatitis or liver cirrhosis. The mean value of S-IRPH was highest in cholestatic diseases, and next highest in acute hepatitis. In addition to acute hepatitis, S-IRPH was increased in other conditions of hepatocellular damage such as exacerbation of chronic hepatitis or immediately after transcatheter arterial embolization of hepatocellular carcinoma. In cases of hepatocellular damage S-IRPH varied concurrent with cytoplasmic enzyme (AST, ALT and LDH) levels and in cases of cholestatic diseases with biliary enzyme (Al-P and gamma GTP) levels. These properties appear to be unique among serum enzymes. The characteristics of S-IRPH were considered to be related to its unique subcellular localization within the cell, ie the membrane of rough endoplasmic reticulum.
采用先前开发的放射免疫分析法，对各类肝病患者血清免疫反应性脯氨酰4-羟化酶（S-IRPH）的浓度进行了测定。急性肝炎（p<0.01）、肝细胞癌（p<0.05）、转移性肝肿瘤（p<0.01）和胆汁淤积性疾病（p<0.001）患者的S-IRPH值升高，但慢性肝炎或肝硬化患者未见明显升高。S-IRPH的平均值在胆汁淤积性疾病中最高，其次是急性肝炎。除急性肝炎外，在其他肝细胞损伤情况下，如慢性肝炎加重或肝细胞癌经导管动脉栓塞术后即刻，S-IRPH也会升高。在肝细胞损伤病例中，S-IRPH与细胞质酶（AST、ALT和LDH）水平同时变化，在胆汁淤积性疾病病例中，S-IRPH与胆汁酶（Al-P和γ-GTP）水平同时变化。这些特性在血清酶中似乎是独特的。S-IRPH的特性被认为与其在细胞内独特的亚细胞定位有关，即粗面内质网的膜。
