Department of Respiratory Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China.
Division of Environmental Health Sciences, The Ohio State University, Columbus, OH, USA.
Inflammation. 2017 Aug;40(4):1214-1224. doi: 10.1007/s10753-017-0564-y.
This study's aim was to investigate the post-effect of an air quality improvement on systemic inflammation and circulating microparticles in asthmatic patients during, and 2 months after, the Beijing Olympics 2008. We measured the levels of circulating inflammatory cytokines and microparticles in the peripheral blood from asthma patients and healthy controls during (phase 1), and 2 months after (phase 2) the Beijing 2008 Olympic Games. The concentrations of circulating cytokines (including TNFα, IL-6, IL-8, and IL-10) were still seen reduced in phase 2 when compared with those in phase 1. The number of circulating endothelial cell-derived microparticles was significantly lower during the phase 2 than that during phase 1 in asthma patients. The level of plasma lipopolysaccharide-binding protein (LBP) was significantly decreased in asthmatics in phase 2. The level of norepinephrine was significantly higher in phase 2 than that in phase 1 in plasma from both asthma patients and healthy subjects. There were no significant differences in the gene profile for the toll-like receptor (TLR) signaling from peripheral blood mononuclear cells. In vitro, microvesicles from patients with asthma impaired the relaxation to bradykinin and contraction to acetylcholine, whereas microparticles from healthy subjects did not. These data suggested that reduction in systemic pro-inflammatory responses and circulating LBP and increased level of norepinephrine in asthma patients persisted even after 2 months of the air pollution intervention. These changes were independent of the TLR signaling pathway. Circulating microparticles might be associated with airway smooth muscle dysfunction.
本研究旨在探讨 2008 年北京奥运会期间及之后空气质量改善对哮喘患者全身炎症反应和循环微粒的后续影响。我们测量了哮喘患者和健康对照者在（第 1 阶段）北京 2008 年奥运会期间和（第 2 阶段）之后 2 个月外周血中循环炎症细胞因子和微粒的水平。与第 1 阶段相比，第 2 阶段循环细胞因子（包括 TNFα、IL-6、IL-8 和 IL-10）的浓度仍降低。与第 1 阶段相比，哮喘患者在第 2 阶段循环内皮细胞衍生微粒的数量显著降低。第 2 阶段哮喘患者血浆脂多糖结合蛋白（LBP）水平显著降低。与哮喘患者和健康受试者的血浆相比，第 2 阶段的去甲肾上腺素水平明显高于第 1 阶段。来自外周血单核细胞的 Toll 样受体（TLR）信号的基因谱没有明显差异。在体外，来自哮喘患者的微囊泡可损害缓激肽的舒张和乙酰胆碱的收缩，而来自健康受试者的微囊泡则不会。这些数据表明，即使在空气污染干预 2 个月后，哮喘患者全身促炎反应、循环 LBP 降低和去甲肾上腺素水平升高仍持续存在。这些变化与 TLR 信号通路无关。循环微粒可能与气道平滑肌功能障碍有关。
