Wang Li-Ren, Cai Le-Yi, Lin Ding-Sheng, Cao Bin, Li Zhi-Jie
*Department of Hand and Plastic Surgery, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China; †School of the First Clinical Medical Sciences, Wenzhou Medical University, Wenzhou, China.
Dermatol Surg. 2017 Oct;43(10):1213-1220. doi: 10.1097/DSS.0000000000001178.
Random skin flaps are commonly used for wound repair and reconstruction. Electroacupuncture at The Zusanli point could enhance microcirculation and blood perfusion in random skin flaps.
To determine whether electroacupuncture at The Zusanli point can improve the survival of random skin flaps in a rat model.
Thirty-six male Sprague Dawley rats were randomly divided into 3 groups: control group (no electroacupuncture), Group A (electroacupuncture at a nonacupoint near The Zusanli point), and Group B (electroacupuncture at The Zusanli point). McFarlane flaps were established. On postoperative Day 2, malondialdehyde (MDA) and superoxide dismutase were detected. The flap survival rate was evaluated, inflammation was examined in hematoxylin and eosin-stained slices, and the expression of vascular endothelial growth factor (VEGF) was measured immunohistochemically on Day 7.
The mean survival area of the flaps in Group B was significantly larger than that in the control group and Group A. Superoxide dismutase activity and VEGF expression level were significantly higher in Group B than those in the control group and Group A, whereas MDA and inflammation levels in Group B were significantly lower than those in the other 2 groups.
Electroacupuncture at The Zusanli point can effectively improve the random flap survival.
随意皮瓣常用于伤口修复与重建。足三里穴位电针可增强随意皮瓣的微循环和血液灌注。
确定足三里穴位电针是否能改善大鼠模型中随意皮瓣的存活情况。
将36只雄性Sprague Dawley大鼠随机分为3组：对照组（无电针）、A组（在足三里穴位附近的非穴位处进行电针）和B组（在足三里穴位进行电针）。制作McFarlane皮瓣。术后第2天，检测丙二醛（MDA）和超氧化物歧化酶。评估皮瓣存活率，苏木精-伊红染色切片检查炎症情况，并在第7天通过免疫组织化学法检测血管内皮生长因子（VEGF）的表达。
B组皮瓣的平均存活面积显著大于对照组和A组。B组超氧化物歧化酶活性和VEGF表达水平显著高于对照组和A组，而B组的MDA和炎症水平显著低于其他两组。
足三里穴位电针可有效提高随意皮瓣的存活率。
