血必净注射液对大鼠随意皮瓣存活的有益作用。
Beneficial effects of Xuebijing injection on random skin flap survival in rats.
作者信息
Bin Cao, Dingsheng Lin, Leyi Cai, Bin Lin, Yuting Lin, Liren Wang, Zhijie Li
机构信息
Department of Hand and Plastic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, The Second Clinical Medical College of Wenzhou Medical University, Wenzhou, China.
Department of Hand and Plastic Surgery, The Second Affiliated Hospital of Wenzhou Medical University, The Second Clinical Medical College of Wenzhou Medical University, Wenzhou, China.
出版信息
J Surg Res. 2015 Jun 15;196(2):421-6. doi: 10.1016/j.jss.2015.03.012. Epub 2015 Mar 19.
BACKGROUND
Improving survival of random skin flaps has important implications of reconstruction, and various kinds of agents have been tested for this purpose. In this study, the effects of Xuebijing (XBJ) injection on random skin flap survival in rats were evaluated.
MATERIALS AND METHODS
McFarlane flaps were established in 40 rats divided into two groups. XBJ was injected into the test group, and the same concentration of saline was injected into controls. Tissues were stained with hematoxylin and eosin stain and immunohistochemically evaluated, and the survival area of the flaps, superoxide dismutase, and malondialdehyde contents were examined.
RESULTS
Compared with the control group, we found the mean survival area of the flaps in the test group was significantly larger, expression of vascular endothelial growth factor and superoxide dismutase activity increased markedly in the test group, malondialdehyde level in the test group was significantly lower, and the hematoxylin and eosin-stained slices showed that inflammation was clearly inhibited in the test group.
CONCLUSIONS
In summary, XBJ improved survival of random skin flaps.
背景
提高随意皮瓣的存活率对重建具有重要意义，为此已对多种药物进行了测试。在本研究中，评估了血必净（XBJ）注射液对大鼠随意皮瓣存活的影响。
材料与方法
将40只大鼠制成麦克法兰皮瓣并分为两组。向试验组注射血必净，向对照组注射相同浓度的生理盐水。对组织进行苏木精-伊红染色并进行免疫组织化学评估，检测皮瓣的存活面积、超氧化物歧化酶和丙二醛含量。
结果
与对照组相比，我们发现试验组皮瓣的平均存活面积显著更大，试验组血管内皮生长因子的表达和超氧化物歧化酶活性明显增加，试验组丙二醛水平显著降低，苏木精-伊红染色切片显示试验组炎症明显受到抑制。
结论
总之，血必净提高了随意皮瓣的存活率。
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