Qi Han, Liu Zheng, Liu Bin, Cao Han, Sun Weiping, Yan Yuxiang, Zhang Ling
Department of Epidemiology and Biostatistics, School of Public Health, Capital Medical University Beijing Municipal Key Laboratory of Clinical Epidemiology Department of Cardiology, Beijing Luhe Hospital, Capital Medical University, Beijing, China.
Medicine (Baltimore). 2017 Apr;96(17):e6417. doi: 10.1097/MD.0000000000006417.
Commonly used tests for diagnosis of salt-sensitive hypertension (SSH) are complex and time-consuming, so new methods are required. Many studies have demonstrated roles for miRNAs in hypertension; however, the diagnostic value of miRNAs has yet to be determined for human SSH. In this study, we examined miRNA expression profiles by initial high-throughput miRNA sequencing of samples from patients with salt-sensitive and salt-resistant hypertension (SSH and SRH, respectively; n = 6, both groups), followed by validation by quantitative real-time polymerase chain reaction (qRT-PCR) in a larger cohort (n = 91). We also evaluated differences in baseline characteristics (e.g., age, sex, body mass index, consumption of specific foods) between the SSH and SRH groups. Of 36 miRNAs identified as differentially expressed between SSH and SRH groups by RNA-Seq, 8 were analyzed by qRT-PCR. There were significant differences in the expression levels of hsa-miR-361-5p and hsa-miR-362-5p between the 2 groups (P = .023 and.049, respectively). In addition, there were significant differences in sauce and poultry consumption between the 2 groups (P = .004 and.001, respectively). The areas under the curve (AUC) determined by receptor operating characteristic (ROC) analysis for hsa-miR-361-5p and all 8 miRNAs were 0.793 (95% CI, 0.698-0.888; sensitivity = 73.9%, specificity = 74.4%; P < .001) and 0.836 (95% CI, 0.749-0.922; sensitivity = 80.4%, specificity = 81.4%; P < .001), respectively, when sauce and poultry consumption were included in the models. Assay feasibility and economic considerations make hsa-miR-361-5p combined with the dietary factors the preferred markers for diagnosis of SSH.
常用的盐敏感性高血压（SSH）诊断测试复杂且耗时，因此需要新的方法。许多研究已证明miRNA在高血压中的作用；然而，miRNA对人类SSH的诊断价值尚未确定。在本研究中，我们首先通过对盐敏感性高血压和盐抵抗性高血压患者（分别为SSH和SRH；每组n = 6）的样本进行高通量miRNA测序来检测miRNA表达谱，随后在更大的队列（n = 91）中通过定量实时聚合酶链反应（qRT-PCR）进行验证。我们还评估了SSH组和SRH组之间基线特征（如年龄、性别、体重指数、特定食物的摄入量）的差异。在通过RNA测序确定的SSH组和SRH组之间差异表达的36种miRNA中，8种通过qRT-PCR进行了分析。两组之间hsa-miR-361-5p和hsa-miR-362-5p的表达水平存在显著差异（分别为P = 0.023和0.049）。此外，两组之间酱汁和家禽的摄入量也存在显著差异（分别为P = 0.004和0.001）。当模型中纳入酱汁和家禽摄入量时，通过受体操作特征（ROC）分析确定的hsa-miR-361-5p和所有8种miRNA的曲线下面积（AUC）分别为0.793（95%CI，0.698 - 0.888；敏感性 = 73.9%，特异性 = 74.4%；P < 0.001）和0.836（95%CI，0.749 - 0.922；敏感性 = 80.4%，特异性 = 81.4%；P < 0.001）。检测可行性和经济因素使得hsa-miR-361-5p联合饮食因素成为诊断SSH的首选标志物。
