Baehr W, Falk J D, Bugra K, Triantafyllos J T, McGinnis J F
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907.
FEBS Lett. 1988 Oct 10;238(2):253-6. doi: 10.1016/0014-5793(88)80490-3.
We have identified three overlapping 5'-truncated mouse opsin cDNA clones by immunologically screening a lambda gt11 retina expression library. Using one of the cDNA clones as a probe, we isolated a 5 kb genomic fragment that encompassed the complete coding sequence for mouse opsin. The coding region for opsin was interrupted by four introns positioned precisely as those previously described for other mammalian opsins. In contrast to the single major opsin mRNA in the bovine and human retina, Northern analysis of mouse retina RNA demonstrated the presence of at least five distinct species of polyadenylated opsin mRNAs. Their sizes ranged from 1.7 kb to 5.1 kb.
我们通过免疫筛选λgt11视网膜表达文库，鉴定出了三个重叠的5'-截短型小鼠视蛋白cDNA克隆。使用其中一个cDNA克隆作为探针，我们分离出了一个5 kb的基因组片段，该片段包含小鼠视蛋白的完整编码序列。视蛋白的编码区域被四个内含子打断，其位置与先前描述的其他哺乳动物视蛋白的内含子位置精确相同。与牛和人类视网膜中单一的主要视蛋白mRNA不同，对小鼠视网膜RNA的Northern分析表明存在至少五种不同的多聚腺苷酸化视蛋白mRNA。它们的大小范围从1.7 kb到5.1 kb。
