Sanchez Diego Noe Rodriguez, Bertanha Matheus, Fernandes Thiago Dias, Resende Luiz Antônio de Lima, Deffune Elenice, Amorim Rogério Martins
Department of Veterinary Clinics, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, SP, Brazil.
Department of Surgery and Orthopedics, Vascular Laboratory, Botucatu Medical School, São Paulo State University (UNESP), Botucatu, SP, Brazil.
Int J Stem Cells. 2017 May 30;10(1):83-92. doi: 10.15283/ijsc16037.
Maintaining a permissive microenvironment is essential for adequate nerve regeneration. Cell-based therapy has the potential based cell replacement and promotion of axonal growth. The adipose tissue derived mesenchymal stromal cells (Ad-MSC) attract interest because neuroregenerative and anti-inflammatory properties. The aim of this study was to evaluate the effects of canine and murine Ad-MSC transplantation on the sciatic nerve regeneration.
Forty Wistar rats were divided randomly into: control group - CG (n=8); denervated group - DG (n=8); decellularized vein group - VG (n=8); decellularized vein＋canine MSC-cMSC (n=8); descellularized vein＋murine MSC-mMSC (n=8). After 10-mm nerve gap, the tubulation technique was performed with decellularized vein filled with 10 MSC labeled with quantum dots (Qtracker 665). The sciatic nerve functional index (SFI) and electroneuromyography (ENMG) measurements were carried and morphometric and immunohistochemistry analysis of the tissue.
The SFI values were higher in the cMSC and mMSC groups at day 27 (p<0.020) and day 35 (p<0.011). The ENMG analysis also revealed better results in the mMSC group. Density, number, and total area of the fibers were increased in the mMSC and cMSC groups. Brain-derived neurotrophic factor BDNF and S-100 protein positive immunoreactivity showed a higher expression for both in the nerve of the mMSC and cMSC groups. The MSC labeled with quantum dots were detected at day 35, indicating neuronal survival long after the nerve damage.
Murine and canine Ad-MSC associated with decellularized vein scaffold had positive effects on sciatic nerve regeneration in rats.
维持一个适宜的微环境对于神经充分再生至关重要。基于细胞的疗法具有细胞替代和促进轴突生长的潜力。脂肪组织来源的间充质基质细胞（Ad-MSC）因其神经再生和抗炎特性而备受关注。本研究旨在评估犬和鼠Ad-MSC移植对坐骨神经再生的影响。
将40只Wistar大鼠随机分为：对照组 - CG（n = 8）；去神经组 - DG（n = 8）；脱细胞静脉组 - VG（n = 8）；脱细胞静脉＋犬MSC - cMSC组（n = 8）；脱细胞静脉＋鼠MSC - mMSC组（n = 8）。在造成10毫米神经缺损后，采用脱细胞静脉填充10个标记有量子点（Qtracker 665）的MSC进行管道技术修复。进行坐骨神经功能指数（SFI）和神经电生理检查（ENMG）测量以及组织的形态计量学和免疫组织化学分析。
在第27天（p < 0.020）和第35天（p < 0.011），cMSC组和mMSC组的SFI值更高。ENMG分析也显示mMSC组结果更好。mMSC组和cMSC组中纤维的密度、数量和总面积增加。脑源性神经营养因子BDNF和S - 100蛋白阳性免疫反应性在mMSC组和cMSC组的神经中均显示出较高表达。在第35天检测到标记有量子点的MSC，表明在神经损伤后很长时间神经元仍存活。
与脱细胞静脉支架联合的鼠和犬Ad-MSC对大鼠坐骨神经再生有积极作用。
