Yang Ying, Xiao Hang, McClements David Julian
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Ministry of Agriculture , Beijing, 100193, China.
Biopolymers and Colloids Research Laboratory, Department of Food Science, University of Massachusetts , Amherst, Massachusetts 01003, United States.
J Agric Food Chem. 2017 May 17;65(19):3946-3955. doi: 10.1021/acs.jafc.7b00955. Epub 2017 May 4.
A simulated gastrointestinal tract/Caco-2 cell culture model was used to investigate the effects of lipid phase type on vitamin E (VE) bioavailability. Oil-in-water emulsions fortified with α-tocopherol acetate were fabricated using a natural emulsifier (quillaja saponin) and long or medium chain triglycerides (LCTs or MCTs) as lipids. The impact of lipid type on VE bioaccessibility, absorption, and transformation was determined. VE bioaccessibility was greater for LCT (46%) than MCT (19%) due to greater solubilization in mixed micelles assembled from longer fatty acids. VE absorption by Caco-2 cells was similar for LCT (28%) and MCT (30%). The transformation of α-tocopherol acetate to α-tocopherol was higher for LCT (90%) than MCT (75%) due to differences in esterase accessibility to VE. Emulsion-based delivery systems formulated using LCT are therefore more suitable for encapsulating and delivering vitamin E than those formulated using MCT.
使用模拟胃肠道/Caco-2细胞培养模型来研究脂质相类型对维生素E（VE）生物利用度的影响。以天然乳化剂（皂树皂苷）和长链或中链甘油三酯（LCTs或MCTs）作为脂质，制备了用醋酸α-生育酚强化的水包油乳液。测定了脂质类型对VE生物可及性、吸收和转化的影响。由于在由较长脂肪酸组装而成的混合胶束中的溶解度更高，LCT的VE生物可及性（46%）高于MCT（19%）。Caco-2细胞对LCT（28%）和MCT（30%）中VE的吸收相似。由于酯酶对VE的可及性存在差异，LCT中醋酸α-生育酚向α-生育酚的转化（90%）高于MCT（75%）。因此，与使用MCT配制的基于乳液的递送系统相比，使用LCT配制的递送系统更适合用于包封和递送维生素E。
