Key Laboratory of Bio-based Material Science and Technology (Ministry of Education), College of Material Science and Engineering , Northeast Forestry University , Harbin , 150040 , People's Republic of China.
Department of Food Science , University of Massachusetts , Amherst , Massachusetts 01003 , United States.
J Agric Food Chem. 2018 Oct 10;66(40):10532-10542. doi: 10.1021/acs.jafc.8b03077. Epub 2018 Oct 1.
In this study, vitamin E was encapsulated in oil-in-water nanoemulsions fabricated using a dual-channel microfluidizer. A long chain triacylglycerol (corn oil) was used as a carrier oil and a biosurfactant (quillaja saponin) was used as a natural emulsifier. The impact of vitamin-to-carrier oil ratio on the formation, storage stability, and bioaccessibility of the nanoemulsions was determined. The lipid droplet size formed during homogenization increased with increasing vitamin content, which was attributed to a large increase in lipid phase viscosity. The storage stability of the nanoemulsions decreased as the vitamin content increased because the larger lipid droplets creamed faster. The rate and extent of lipid hydrolysis in the small intestine decreased as the vitamin content increased, probably because the vitamin molecules inhibited the ability of lipase to reach the triacylglycerols inside the lipid droplets. Vitamin bioaccessibility decreased as the vitamin level in the lipid phase increased, which was attributed to the reduced level of mixed micelles available to solubilize the tocopherols. The optimized nanoemulsion-based delivery system led to a relatively high vitamin bioaccessibility (53.9%). This research provides valuable information for optimizing delivery systems to increase the bioaccessibility of oil-soluble vitamins.
在这项研究中，维生素 E 被包封在使用双通道微流体制备的油包水型纳米乳液中。长链三酰基甘油（玉米油）用作载体油，生物表面活性剂（皂树皮）用作天然乳化剂。确定了维生素与载体油比对于纳米乳液的形成、储存稳定性和生物利用度的影响。均质过程中形成的脂质液滴大小随维生素含量的增加而增加，这归因于脂质相粘度的大幅增加。纳米乳液的储存稳定性随维生素含量的增加而降低，因为较大的脂质液滴更快地形成乳状液。由于维生素分子抑制了脂肪酶到达脂质滴内三酰基甘油的能力，因此在小肠中脂质水解的速率和程度随维生素含量的增加而降低。随着脂质相中维生素水平的增加，维生素的生物利用度降低，这归因于可用于溶解生育酚的混合胶束水平降低。优化的基于纳米乳液的递药系统导致相对较高的维生素生物利用度（53.9%）。这项研究为优化递药系统以提高油溶性维生素的生物利用度提供了有价值的信息。
