Yang Fengmei, Gao Bo, Li Rui, Li Wencui, Chen Wei, Yu Zongtao, Zhang Jicai
Department of Obstetrics and Gynecology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
Department of Laboratory Medicine, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
Mol Med Rep. 2017 May;15(5):2802-2806. doi: 10.3892/mmr.2017.6328. Epub 2017 Mar 15.
Tumor cells may develop multidrug resistance (MDR) to various chemotherapy regimens. Such resistance reduces the sensitivity of cells to chemotherapy drugs, leading to the failure of cervical cancer (CC) treatment and disease progression. The present study aimed to investigate the role of MDR1, lung resistance protein (LRP) and placental glutathione S‑transferase π 1 (GSTP1) in CC and MDR, and the prognostic value of these genes. The mRNA expression levels of these resistance‑associated genes were determined in 47 CC and 20 healthy cervical tissue samples. Subsequently, the data was analyzed alongside clinicopathological parameters. The mRNA expression levels of MDR1, LRP and GSTP1 in CC were 0.57±0.32, 0.58±0.29 and 0.44±0.24, respectively, whereas those in healthy cervical tissues were 0.19±0.10, 0.17±0.14 and 0.18±0.10, respectively. Therefore, the expression levels of these genes were significantly greater in CC compared with healthy cervical tissue (P<0.05). mRNA expression levels of MRD1 were increased in the well differentiated group (0.68±0.27) compared with the poorly differentiated group (0.38±0.33; P<0.05). No significant differences were observed between LRP and GSTP1 mRNA expression levels and tumor differentiation or clinical stage of the patients (P>0.05). Multivariate logistic regression indicated that the degree of differentiation and the MDR1 gene expression levels were predictors of CC prognosis (P<0.05). The survival rate of patients in the MDR1‑negative group was significantly greater compared with the MDR1‑positive group (P<0.05). The results of the present study therefore suggested that MDR1 gene expression is a predictor of poor survival in CC.
肿瘤细胞可能对各种化疗方案产生多药耐药性（MDR）。这种耐药性会降低细胞对化疗药物的敏感性，导致宫颈癌（CC）治疗失败和疾病进展。本研究旨在探讨多药耐药蛋白1（MDR1）、肺耐药蛋白（LRP）和胎盘型谷胱甘肽S-转移酶π1（GSTP1）在CC和MDR中的作用，以及这些基因的预后价值。在47例CC组织样本和20例健康宫颈组织样本中测定了这些耐药相关基因的mRNA表达水平。随后，将数据与临床病理参数一起进行分析。CC组织中MDR1、LRP和GSTP1的mRNA表达水平分别为0.57±0.32、0.58±0.29和0.44±0.24，而健康宫颈组织中的表达水平分别为0.19±0.10、0.17±0.14和0.18±0.10。因此，与健康宫颈组织相比，CC组织中这些基因的表达水平显著更高（P<0.05）。与低分化组（0.38±0.33）相比，高分化组（0.68±0.27）中MRD1的mRNA表达水平升高（P<0.05）。未观察到LRP和GSTP1 mRNA表达水平与患者肿瘤分化程度或临床分期之间存在显著差异（P>0.05）。多因素逻辑回归分析表明，分化程度和MDR1基因表达水平是CC预后的预测指标（P<0.05）。MDR1阴性组患者的生存率显著高于MDR1阳性组（P<0.05）。因此，本研究结果表明，MDR1基因表达是CC患者生存不良的预测指标。
