Chizenga Elvin Peter, Chandran Rahul, Abrahamse Heidi
Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, Johannesburg, South Africa.
Oncotarget. 2019 Jul 9;10(43):4380-4396. doi: 10.18632/oncotarget.27029.
The heterogeneous nature of cancer puts cancer stem cells (CSCs) at the beating heart of the tumour. Because of their inherent characteristics of stemness, CSCs evade putative cancer therapies, resulting in treatment resistance or tumour recurrence after a seemingly successful treatment. To prevent treatment resistance and cancer recurrence, killing the beating heart of the tumour is of utmost importance. This study therefore, sought to determine the effect of Photodynamic Therapy (PDT) in eradicating cervical cancer and cervical CSCs. Cervical CSCs were isolated from a cervical adenocarcinoma cell line, HeLa cells, and grown in liquid medium incubated at 37° C, 5% CO and 85% humidity. Increasing doses of AlPcS photosensitizer were administered to both the total cell population and the isolated CSCs, and irradiated using 673.2 nm diode laser. Post-irradiation cellular changes were observed using biochemical assays and microscopy to determine the response of both the total cell population and the CSCs. Results showed a dose-dependent response of both cell populations to treatment, by demonstration of significant morphologic changes, increased cytotoxicity, and decreased cell viability and proliferation. The study suggested that PDT using AlPcS is a very effective treatment method for the eradication of cervical cancer cells and cervical CSCs, .
癌症的异质性使癌症干细胞（CSCs）成为肿瘤的核心。由于其固有的干性特征，癌症干细胞能够逃避既定的癌症治疗方法，导致在看似成功的治疗后出现治疗抵抗或肿瘤复发。为了防止治疗抵抗和癌症复发，杀死肿瘤的核心至关重要。因此，本研究旨在确定光动力疗法（PDT）在根除宫颈癌和宫颈癌症干细胞方面的效果。从宫颈腺癌细胞系HeLa细胞中分离出宫颈癌症干细胞，并在37°C、5%二氧化碳和85%湿度条件下培养的液体培养基中培养。对总细胞群体和分离出的癌症干细胞均给予递增剂量的磺化铝酞菁（AlPcS）光敏剂，并使用673.2纳米二极管激光进行照射。照射后，通过生化分析和显微镜观察细胞变化，以确定总细胞群体和癌症干细胞的反应。结果显示，通过显著的形态学变化、细胞毒性增加以及细胞活力和增殖减少，两个细胞群体对治疗均呈现剂量依赖性反应。该研究表明，使用磺化铝酞菁的光动力疗法是根除宫颈癌细胞和宫颈癌症干细胞的一种非常有效的治疗方法。
