a Epigenetics & Cell Fate Centre, UMR7216 CNRS , Paris Diderot University, Sorbonne Paris Cité , Paris , France.
Nucleus. 2017 May 4;8(3):255-260. doi: 10.1080/19491034.2017.1292192. Epub 2017 Feb 23.
In the cell nucleus, DNA repair machineries operate on a chromatin substrate, whose integrity is key for preserving cell functions and identity. Yet, it is still unclear how the epigenetic information conveyed by chromatin is maintained during the DNA repair process. We recently characterized the dynamics of parental histones coupled to UV-C damage repair in human cells, providing insights into how the pre-damage chromatin state may be restored. Here, we summarize our main findings and discuss them in the context of epigenome maintenance following DNA damage. We further address the mechanistic aspects of repair-coupled histone dynamics and develop working hypotheses regarding their functional relevance in the cellular response to genotoxic stress.
在细胞核中，DNA 修复机制在染色质底物上发挥作用，其完整性对于维持细胞功能和身份至关重要。然而，目前尚不清楚染色质所传递的表观遗传信息如何在 DNA 修复过程中得以维持。我们最近对人类细胞中与 UV-C 损伤修复相关的亲本组蛋白动力学进行了表征，深入了解了预先存在的染色质状态如何得以恢复。在这里，我们总结了主要发现，并在 DNA 损伤后表观基因组维持的背景下对其进行了讨论。我们进一步探讨了修复相关组蛋白动力学的机制方面，并提出了关于其在细胞应对遗传毒性应激中的功能相关性的工作假设。
