Tailoring of TiO films by HSO treatment and UV irradiation to improve anticoagulant ability and endothelial cell compatibility.

Surfaces with dual functions that simultaneously exhibit good anticoagulant ability and endothelial cell (EC) compatibility are desirable for blood contact materials. However, these dual functions have rarely been achieved by inorganic materials. In this study, titanium dioxide (TiO) films were treated by sulphuric acid (HSO) and ultraviolet (UV) irradiation successively (TiOHSO-UV), resulting in good anticoagulant ability and EC compatibility simultaneously. We found that UV irradiation improved the anticoagulant ability of TiO films significantly while enhancing EC compatibility, though not significantly. The enhanced anticoagulant ability could be related to the oxidation of surface-adsorbed hydrocarbons and increased hydrophilicity. The HSO treatment improved the anticoagulant ability of TiO films slightly, while UV irradiation improved the anticoagulant ability strongly. The enhanced EC compatibility could be related to the increased surface roughness and positive charges on the surface of the TiO films. Furthermore, the time-dependent degradation of the enhanced EC compatibility and anticoagulant ability of TiOHSO-UV was observed. In summary, TiOHSO-UV expressed both excellent anticoagulant ability and good EC compatibility at the same time, which could be desirable for blood contact materials. However, the compatibility of TiOHSO-UV with smooth muscle cells (SMCs) and macrophages was also improved. More effort is still needed to selectively improve EC compatibility on TiO films for better re-endothelialization.