Rinaldi Stefano, Pallikkuth Suresh, George Varghese K, de Armas Lesley R, Pahwa Rajendra, Sanchez Celeste M, Pallin Maria Fernanda, Pan Li, Cotugno Nicola, Dickinson Gordon, Rodriguez Allan, Fischl Margaret, Alcaide Maria, Gonzalez Louis, Palma Paolo, Pahwa Savita
Miami Center for AIDS Research, Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Academic Department of Pediatrics (DPUO), Research Unit in Congenital and Perinatal Infections, Bambino Gesù Children's Hospital-University of Rome Tor Vergata, Rome, Italy.
Aging (Albany NY). 2017 Apr;9(4):1307-1325. doi: 10.18632/aging.101229.
Combination antiretroviral therapies (cART)can lead to normal life expectancy in HIV-infected persons, and people aged >50 yrs represent the fastest growing HIV group. Although HIV and aging are independently associated with impaired humoral immunity, immune status in people aging with HIV is relatively unexplored. In this study influenza vaccination was used to probe age associated perturbations in the B cell compartment of HIV-negative "healthy controls" (HC) and virologically controlled HIV-infected participants on cART (HIV) (n=124), grouped by age as young (<40 yrs), middle-aged (40-59yrs) or old (60 yrs). H1N1 antibody response at d21 post-vaccination correlated inversely with age in both HC and HIV. Immunophenotyping of cryopreserved PBMC demonstrated increased frequencies of double negative B cells and decreased plasmablasts in old compared to young HC. Remarkably, young HIV were different from young HC but similar to old HC in B cell phenotype, influenza specific spontaneous (d7) or memory (d21) antibody secreting cells. We conclude that B cell immune senescence is a prominent phenomenon in young HIV in comparison to young HC, but distinctions between old HIV and old HC are less evident though both groups manifest age-associated B cell dysfunction.
联合抗逆转录病毒疗法（cART）可使HIV感染者的预期寿命正常化，50岁以上人群是增长最快的HIV群体。虽然HIV感染和衰老各自都与体液免疫受损相关，但HIV感染者衰老过程中的免疫状态相对缺乏研究。在本研究中，流感疫苗接种被用于探究在接受cART治疗的HIV阴性“健康对照”（HC）和病毒学得到控制的HIV感染者（HIV）（n = 124）的B细胞区室中与年龄相关的扰动，按年龄分为年轻组（<40岁）、中年组（40 - 59岁）或老年组（≥60岁）。接种疫苗后第21天的H1N1抗体反应在HC组和HIV组中均与年龄呈负相关。对冻存的外周血单个核细胞（PBMC）进行免疫表型分析显示，与年轻HC组相比，老年HC组中双阴性B细胞频率增加，浆母细胞减少。值得注意的是，年轻HIV感染者在B细胞表型、流感特异性自发（第7天）或记忆（第21天）抗体分泌细胞方面与年轻HC组不同，但与老年HC组相似。我们得出结论，与年轻HC组相比，年轻HIV感染者中B细胞免疫衰老现象显著，但老年HIV感染者和老年HC组之间的差异不太明显，尽管两组均表现出与年龄相关的B细胞功能障碍。
