Teunissen M B, Wormmeester J, Kapsenberg M L, Bos J D
Department of Dermatology, University of Amsterdam, The Netherlands.
J Invest Dermatol. 1988 Oct;91(4):358-62. doi: 10.1111/1523-1747.ep12475770.
In this report we introduce an alternative procedure for enrichment of human epidermal Langerhans cells (LC) from epidermal cell suspensions of normal skin. By means of discontinuous Ficoll-Metrizoate density gradient centrifugation, a fraction containing high numbers of viable, more than 80% pure LC was recovered, as judged by CD1a expression. The purity of the LC-enriched fraction appeared to be dependent on the percentage LC in the crude epidermal cell suspension. LC enriched by this method retained their accessory and antigen-presenting capacities, as determined in the Concanavalin-A induced T-cell response, in the allogeneic mixed leukocyte reaction and in the antigen-specific T-cell proliferation assay in vitro. The great advantage of this method is that it is simple and rapid and that the isolated LC are unlabeled.
在本报告中，我们介绍了一种从正常皮肤的表皮细胞悬液中富集人表皮朗格汉斯细胞（LC）的替代方法。通过不连续的菲可-泛影葡胺密度梯度离心，回收了一个含有大量存活的、纯度超过80%的LC的组分，这是通过CD1a表达判断的。富含LC的组分的纯度似乎取决于粗表皮细胞悬液中LC的百分比。通过这种方法富集的LC保留了它们的辅助和抗原呈递能力，这在体外刀豆蛋白A诱导的T细胞反应、同种异体混合淋巴细胞反应和抗原特异性T细胞增殖试验中得到了确定。这种方法的一大优点是简单快速，而且分离出的LC未被标记。
