Vannier J P, Monconduit M, Piguet H
Biomedicine. 1980 Nov;33(7):236-9.
We separated 40 bone marrow samples by centrifugation on two density gradients, ficoll-metrizoate sodium (lymphoprep) and percoll. There is no difference between the two methods when expressing the colony forming cells (CFU-C) per 2.10(5) bone marrow cells plated. But the absolute number of recovered CFU-C is significantly greater with percoll than with ficoll metrizoate sodium.
我们通过在两种密度梯度(菲可-泛影葡胺钠(淋巴细胞分离液)和 Percoll)上进行离心,对 40 份骨髓样本进行了分离。当以每接种 2×10⁵ 个骨髓细胞的集落形成细胞(CFU-C)来表示时,两种方法之间没有差异。但是,Percoll 法回收的 CFU-C 的绝对数量明显多于菲可-泛影葡胺钠法。