Duell E A, Ellis C N, Voorhees J J
Department of Dermatology, University of Michigan Medical School, Ann Arbor 48109-0528.
J Invest Dermatol. 1988 Nov;91(5):446-50. doi: 10.1111/1523-1747.ep12476562.
Keratomed epidermal tissue from normal individuals and from the lesional and non-lesional skin of psoriasis patients served as source materials for the extraction, separation, and quantitation of eicosanoids that may be important to cutaneous function and pathophysiology. The eicosanoids were extracted in ethanol and buffer, partially purified on SEP-PAKs, separated by reverse phase microbore high-performance liquid chromatography, and quantitated by radioimmunoassay or integration of absorbency peaks obtained by high performance liquid chromatography. The involved areas from psoriasis patients contained a statistically significant seven- to 11-fold increase in the levels of leukotriene B4, 13-hydroxyoctadecadienoic acid-like compound, 15-hydroxyeicosatetraenoic acid compound and 12-hydroxyeicosatetraenoic acid in comparison to normal samples and a three- to sevenfold increase in comparison to uninvolved tissue. The uninvolved areas contained 40% to 100% increases in the levels of these compounds in comparison to normal tissue; these increases were statistically significant except for 13-hydroxyoctadecadienoic acid-like compound. From a single keratome biopsy, multiple eicosanoids can be separated and quantitated; in addition, levels before, during, and after therapy for psoriasis may be compared.
来自正常个体以及银屑病患者皮损和非皮损皮肤的角膜刀切割的表皮组织，用作提取、分离和定量对皮肤功能和病理生理学可能重要的类二十烷酸的原材料。类二十烷酸在乙醇和缓冲液中提取，在SEP-PAKs上部分纯化，通过反相微孔高效液相色谱分离，并通过放射免疫测定或高效液相色谱获得的吸收峰积分进行定量。与正常样本相比，银屑病患者的受累区域白三烯B4、13-羟基十八碳二烯酸样化合物、15-羟基二十碳四烯酸化合物和12-羟基二十碳四烯酸水平有统计学意义的7至11倍增加，与未受累组织相比增加3至7倍。与正常组织相比，未受累区域这些化合物的水平增加40%至100%；除13-羟基十八碳二烯酸样化合物外，这些增加具有统计学意义。从单次角膜刀活检中，可以分离和定量多种类二十烷酸；此外，可以比较银屑病治疗前、治疗期间和治疗后的水平。
