Nagai N, Kioka H, Shigemasa K, Shinko Y, Fujiwara A
Department of Obstetrics and Gynecology, Hiroshima School of Medicine.
Nihon Sanka Fujinka Gakkai Zasshi. 1988 Sep;40(9):1393-9.
In order to investigate the relationship between the presence of human papillomavirus (HPV) DNA and cervical carcinoma, we examined the cervical screening cells as well as the biopsy specimens obtained from 3 cases of severe dysplasia, 13 cases of carcinoma in situ (CIS) and 2 cases of microinvasive carcinoma for the presence of HPV types 6, 11, 16 and 18 DNA by DNA-DNA in situ hybridization using the biotinylated HPV DNA probes. The results of in situ hybridization analysis revealed that HPV 16 DNA sequences were detected in the nuclei of koilocytosis of severe dysplasia and CIS cases. The nuclei of atypical cells obtained from cervical screening cells were positive for HPV 16 or 18 DNA sequences. Two CIS cases were positive for the presence of HPV 16 and 18 DNA sequences. None of them contained HPV 6/11 DNA sequences. Eighteen cervical screening cases were examined and 10 contained HPV 16 DNA sequences and 6 contained HPV 18 DNA sequences. We suggest that the identification of HPV DNA types in cervical screening cells by in situ hybridization might be of diagnostic and prognostic value in early cervical neoplasia.
为了研究人乳头瘤病毒（HPV）DNA的存在与宫颈癌之间的关系，我们通过使用生物素化的HPV DNA探针的DNA-DNA原位杂交，检测了3例重度发育异常、13例原位癌（CIS）和2例微浸润癌的宫颈筛查细胞以及活检标本中HPV 6、11、16和18型DNA的存在情况。原位杂交分析结果显示，在重度发育异常和CIS病例的挖空细胞的细胞核中检测到HPV 16 DNA序列。从宫颈筛查细胞获得的非典型细胞的细胞核中HPV 16或18 DNA序列呈阳性。2例CIS病例HPV 16和18 DNA序列呈阳性。它们均不含有HPV 6/11 DNA序列。对18例宫颈筛查病例进行了检测，其中10例含有HPV 16 DNA序列，6例含有HPV 18 DNA序列。我们认为，通过原位杂交鉴定宫颈筛查细胞中的HPV DNA类型可能对早期宫颈肿瘤具有诊断和预后价值。
