Walters R G, Wilbraham H O, Strike P, Forster J W
Department of Genetics, Liverpool University, UK.
J Gen Microbiol. 1988 Feb;134(2):403-12. doi: 10.1099/00221287-134-2-403.
The formation of single-stranded breaks in DNA following UV irradiation is assessed in uvrC34 mutants. By altering the SOS DNA-repair system, either by additional mutations or by using drugs affecting transcription or translation, it is shown that such single-stranded breaks require one or more DNA-damage-inducible functions. A UV-sensitive strain is characterized as carrying a Tn10 insertion into the uvrC gene. The absence of post-irradiation incision in this strain demonstrates that uvrC function is absolutely required in vivo for the incision stage of excision repair, and suggests that other uvrC mutants are 'leaky'.
在uvrC34突变体中评估紫外线照射后DNA中单链断裂的形成情况。通过额外的突变或使用影响转录或翻译的药物来改变SOS DNA修复系统，结果表明这种单链断裂需要一种或多种DNA损伤诱导功能。一种对紫外线敏感的菌株被鉴定为在uvrC基因中携带一个Tn10插入片段。该菌株在照射后没有切口，这表明uvrC功能在体内对于切除修复的切口阶段是绝对必需的，并表明其他uvrC突变体是“渗漏的”。
