[欧文氏菊黄杆菌ENA49 uvr基因在大肠杆菌K12细胞中的表达]
[Expression of Erwinia chrysanthemi ENA49 uvr gene in Escherichia coli K12 cells].
作者信息
Prokulevich V A, Fomichev Iu K
出版信息
Mol Gen Mikrobiol Virusol. 1988 Jul(7):24-6.
The uvrA gene of Erwinia chrysanthemi ENA49 similar to uvrA gene of Escherichia coli K12 has been cloned in vivo in Escherichia coli AB1886 uvrA6 cells using the plasmid pULB113 (RP4mini Mu). The presence of pULB113 carrying uvrA gene of Erwinia in Escherichia coli K12 uvrA- cells resulted in suppression of this mutation while uvrB and uvrC are not suppressed by this locus. The genetic control of excision repair of UV-damage in Erwinia chrysanthemi ENA49 is concluded to be similar to the one in Escherichia coli K12.
利用质粒pULB113（RP4mini Mu），已在体内将与大肠杆菌K12的uvrA基因相似的菊欧文氏菌ENA49的uvrA基因克隆到大肠杆菌AB1886 uvrA6细胞中。在大肠杆菌K12 uvrA-细胞中携带菊欧文氏菌uvrA基因的pULB113的存在导致该突变的抑制，而uvrB和uvrC不被该位点抑制。得出结论，菊欧文氏菌ENA49中紫外线损伤切除修复的遗传控制与大肠杆菌K12中的相似。
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