单纯疱疹病毒编码的核糖核苷酸还原酶是该病毒在小鼠中致病性的决定因素，也是一个有效的抗病毒靶点。

Ribonucleotide reductase encoded by herpes simplex virus is a determinant of the pathogenicity of the virus in mice and a valid antiviral target.

作者信息

Cameron J M, McDougall I, Marsden H S, Preston V G, Ryan D M, Subak-Sharpe J H

机构信息

Glaxo Group Research Limited, Greenford, Middlesex, U.K.

出版信息

J Gen Virol. 1988 Oct;69 ( Pt 10):2607-12. doi: 10.1099/0022-1317-69-10-2607.

DOI:10.1099/0022-1317-69-10-2607

PMID:2844969

Abstract

The role of the herpes simplex virus (HSV)-encoded ribonucleotide reductase (RR) in the pathogenicity of the virus has been examined by use of mutants with lesions in either the large or small subunit of the enzyme. The virulence of the mutants in mice was reduced by about 10(6)-fold when compared with that of the parental virus (HSV type 1 strain 17), while the virulence of a revertant of one of the mutants was restored to within about 100-fold of that of the parent virus. These experiments demonstrate that activity of the HSV RR is essential for virus pathogenicity in mice and suggests that the enzyme is a valid target for specific antiviral compounds.

摘要

通过使用在该酶的大亚基或小亚基中存在损伤的突变体，研究了单纯疱疹病毒（HSV）编码的核糖核苷酸还原酶（RR）在病毒致病性中的作用。与亲本病毒（HSV 1型毒株17）相比，这些突变体在小鼠中的毒力降低了约10^6倍，而其中一个突变体的回复株的毒力恢复到亲本病毒的约100倍以内。这些实验表明，HSV RR的活性对于小鼠中的病毒致病性至关重要，并表明该酶是特异性抗病毒化合物的有效靶点。