Saad E S, Milley K M, Al-Khan A A, Nimmo J S, Bacci B, Tayebi M, Day M J, Richardson S J, Danks J A
School of Health and Biomedical Sciences, RMIT University, Bundoora, Victoria, Australia.
Australian Specialised Animal Pathology Laboratory, Mulgrave, Victoria, Australia.
J Comp Pathol. 2017 May;156(4):352-365. doi: 10.1016/j.jcpa.2017.03.005. Epub 2017 Apr 24.
Canine mixed mammary tumours (CMMTs) and human metaplastic breast carcinomas (HMBCs) share several histopathological features and risk factors. In both species, these tumours display epithelial and stromal components. HMBCs are rare malignant tumours, but CMMTs are one of the most common mammary tumours in dogs and are more often benign than malignant. In this study, benign (n = 88) and malignant (n = 13) CMMTs were characterized using specific antibodies against oestrogen receptor, progesterone receptor, human epidermal growth factor receptor 2, cytokeratin 5/6, cytokeratin AE1/AE3, vimentin, Ki67, E-cadherin and p63. Cartilage and bone matrices associated with benign and malignant CMMTs were characterized using specific antibodies against BMP4, Runx2, Sox9 and osteopontin. The current study suggested that CMMTs are of epithelial origin, but display a myoepithelial-like differentiation. The findings suggest key roles for Sox9, Runx2 and BMP4 in chondrogenesis and bone formation in CMMTs. The high expression of osteopontin in CMMTs appears to be unrelated to tumour malignancy.
犬混合性乳腺肿瘤（CMMTs）和人化生性乳腺癌（HMBCs）具有一些共同的组织病理学特征和风险因素。在这两个物种中，这些肿瘤均表现出上皮和间质成分。HMBCs是罕见的恶性肿瘤，但CMMTs是犬类最常见的乳腺肿瘤之一，且良性肿瘤比恶性肿瘤更为常见。在本研究中，使用针对雌激素受体、孕激素受体、人表皮生长因子受体2、细胞角蛋白5/6、细胞角蛋白AE1/AE3、波形蛋白、Ki67、E-钙黏蛋白和p63的特异性抗体对良性（n = 88）和恶性（n = 13）CMMTs进行了表征。使用针对BMP4、Runx2、Sox9和骨桥蛋白的特异性抗体对与良性和恶性CMMTs相关的软骨和骨基质进行了表征。当前研究表明，CMMTs起源于上皮，但表现出肌上皮样分化。研究结果表明Sox9、Runx2和BMP4在CMMTs的软骨形成和骨形成中起关键作用。CMMTs中骨桥蛋白的高表达似乎与肿瘤恶性程度无关。
