综合活细胞成像分析 cryptotanshinone 和协同药物筛选对各种人源和犬源癌细胞系的作用。
Comprehensive live-cell imaging analysis of cryptotanshinone and synergistic drug-screening effects in various human and canine cancer cell lines.
机构信息
Center for Bioinformatics and Genomic Systems Engineering, Texas A&M Engineering Experiment Station, Texas A&M University, College Station, TX, United States of America.
Translational Genomics Research Institute, Phoenix, AZ, United States of America.
出版信息
PLoS One. 2021 Feb 5;16(2):e0236074. doi: 10.1371/journal.pone.0236074. eCollection 2021.
BACKGROUND
Several studies have highlighted both the extreme anticancer effects of Cryptotanshinone (CT), a Stat3 crippling component from Salvia miltiorrhiza, as well as other STAT3 inhibitors to fight cancer.
METHODS
Data presented in this experiment incorporates 2 years of in vitro studies applying a comprehensive live-cell drug-screening analysis of human and canine cancer cells exposed to CT at 20 μM concentration, as well as to other drug combinations. As previously observed in other studies, dogs are natural cancer models, given to their similarity in cancer genetics, epidemiology and disease progression compared to humans.
RESULTS
Results obtained from several types of human and canine cancer cells exposed to CT and varied drug combinations, verified CT efficacy at combating cancer by achieving an extremely high percentage of apoptosis within 24 hours of drug exposure.
CONCLUSIONS
CT anticancer efficacy in various human and canine cancer cell lines denotes its ability to interact across different biological processes and cancer regulatory cell networks, driving inhibition of cancer cell survival.
背景
多项研究强调了丹参酮（CT）的抗癌作用，它是丹参中的 Stat3 抑制剂，其他 STAT3 抑制剂也具有抗癌作用。
方法
本实验中的数据整合了 2 年的体外研究，应用综合的活细胞药物筛选分析，研究了浓度为 20μM 的 CT 对人类和犬科癌细胞的作用，以及其他药物组合的作用。如前所述，由于犬科动物在癌症遗传学、流行病学和疾病进展方面与人类相似，因此它们是天然的癌症模型。
结果
将 CT 和不同药物组合暴露于多种类型的人类和犬科癌细胞中，结果表明 CT 在 24 小时的药物暴露后，通过实现极高比例的细胞凋亡来有效对抗癌症。
结论
CT 在各种人类和犬科癌细胞系中的抗癌效果表明，它能够在不同的生物学过程和癌症调控细胞网络中相互作用，抑制癌细胞的存活。
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