Lin Si-Fang, Wang Bing, Zhang Feng-Ming, Fei Yuan-Hui, Gu Jia-Hui, Li Jie, Bi Ling-Bo, Liu Xing-Jun
Department of Anesthesiology, China-Japan Friendship Hospital, Beijing, 100029, China; Jiangsu Province Key Laboratory of Anesthesiology, School of Anesthesiology, Xuzhou Medical University, Xuzhou, Jiangsu, 221004, China.
Jiangsu Province Key Laboratory of Anesthesiology, School of Anesthesiology, Xuzhou Medical University, Xuzhou, Jiangsu, 221004, China.
Biochem Biophys Res Commun. 2017 Jun 10;487(4):801-806. doi: 10.1016/j.bbrc.2017.04.127. Epub 2017 Apr 24.
T-type calcium channels are prominently expressed in primary nociceptive fibers and well characterized in pain processes. Although itch and pain share many similarities including primary sensory fibers, the function of T-type calcium channels on acute itch has not been explored. We investigated whether T-type calcium channels expressed within primary sensory fibers of mouse skin, especially Ca3.2 subtype, involve in chloroquine-, endothelin-1- and histamine-evoked acute itch using pharmacological, neuronal imaging and behavioral analyses. We found that pre-locally blocking three subtypes of T-type calcium channels in the peripheral afferents of skins, yielded an inhibition in acute itch or pain behaviors, while selectively blocking the Ca3.2 channel in the skin peripheral afferents only inhibited acute pain but not acute itch. These results suggest that T-type Ca3.1 or Ca3.3, but not Ca3.2 channel, have an important role in acute itch processing, and their distinctive roles in modulating acute itch are worthy of further investigation.
T型钙通道在初级伤害性感受纤维中大量表达，并且在疼痛过程中具有明确的特征。尽管瘙痒和疼痛有许多相似之处，包括初级感觉纤维，但T型钙通道在急性瘙痒中的作用尚未得到探索。我们使用药理学、神经元成像和行为分析方法，研究了小鼠皮肤初级感觉纤维中表达的T型钙通道，特别是Ca3.2亚型，是否参与氯喹、内皮素-1和组胺诱发的急性瘙痒。我们发现，预先局部阻断皮肤外周传入神经中的三种T型钙通道亚型，可抑制急性瘙痒或疼痛行为，而仅选择性阻断皮肤外周传入神经中的Ca3.2通道，仅抑制急性疼痛而非急性瘙痒。这些结果表明，T型Ca3.1或Ca3.3通道而非Ca3.2通道，在急性瘙痒处理中起重要作用，它们在调节急性瘙痒中的独特作用值得进一步研究。
