一种类似拟南芥PYL1的蛋白质BrPYL1作为白菜中假定的脱落酸受体的分子特征分析

Molecular characterization of an AtPYL1-like protein, BrPYL1, as a putative ABA receptor in Brassica rapa.

作者信息

Li Yanlin, Wang Dandan, Sun Congcong, Hu Xiaochen, Mu Xiaoqian, Hu Jingjiang, Yang Yongqing, Zhang Yanfeng, Xie Chang Gen, Zhou Xiaona

机构信息

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling 712100, Shaanxi, China.

State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100091, China.

出版信息

Biochem Biophys Res Commun. 2017 Jun 3;487(3):684-689. doi: 10.1016/j.bbrc.2017.04.116. Epub 2017 Apr 24.

DOI:10.1016/j.bbrc.2017.04.116

PMID:28450111

Abstract

Abscisic acid (ABA)-induced physiological changes are conserved in many land plants and underlie their responses to environmental stress and pathogens. The PYRABACTIN RESISTANCE1/PYR1-LIKE/REGULATORY COMPONENTS OF ABA RECEPTORS (PYLs)-type receptors perceive the ABA signal and initiate signal transduction. Here, we show that the genome of Brassica rapa encodes 24 putative AtPYL-like proteins. The AtPYL-like proteins in Brassica rapa (BrPYLs) can also be classified into 3 subclasses. We found that nearly all BrPYLs displayed high expression in at least one tissue. Overexpression of BrPYL1 conferred ABA hypersensitivity to Arabidopsis. Further, ABA activated the expression of an ABA-responsive reporter in Arabidopsis protoplasts expressing BrPYL1. Overall, these results suggest that BrPYL1 is a putative functional ABA receptor in Brassica rapa.

摘要

脱落酸（ABA）诱导的生理变化在许多陆地植物中是保守的，并且是它们对环境胁迫和病原体作出反应的基础。PYRABACTIN RESISTANCE1/PYR1-LIKE/ABA受体的调节成分（PYLs）型受体感知ABA信号并启动信号转导。在此，我们表明，白菜基因组编码24种假定的AtPYL样蛋白。白菜中的AtPYL样蛋白（BrPYLs）也可分为3个亚类。我们发现几乎所有的BrPYLs在至少一种组织中都表现出高表达。BrPYL1的过表达使拟南芥对ABA超敏感。此外，ABA激活了在表达BrPYL1的拟南芥原生质体中ABA响应报告基因的表达。总体而言，这些结果表明BrPYL1是白菜中一种假定的功能性ABA受体。