Genome-Wide Analysis of the PYL Gene Family and Identification of PYL Genes That Respond to Abiotic Stress in Brassica napus.

Abscisic acid (ABA) is an endogenous phytohormone that plays important roles in the regulation of plant growth, development, and stress responses. The pyrabactin resistance 1-like (PYR/PYL) protein is a core regulatory component of ABA signaling networks in plants. However, no details regarding this family in are available. Here, 46 were identified in the genome. Based on phylogenetic analysis, and belong to subfamily I, belong to subfamily II, and and belong to subfamily III. Analysis of conserved motifs showed that every subfamily contained four common motifs. By predicting cis-elements in the promoters, we found that all members contained hormone- and stress-related elements and that expression levels of most were relatively higher in seeds at the germination stage than those in other organs or at other developmental stages. Gene Ontology (GO) enrichment showed that genes mainly participate in responses to stimuli. To identify crucial mediating the response to abiotic stress in , expression changes in 14 genes were determined by quantitative real-time RT-PCR after drought, heat, and salinity treatments, and identified , , and in respond to abiotic stresses. The findings of this study lay a foundation for further investigations of genes in .