McGuigan John A S, Kay James W, Elder Hugh Y
Medical, Veterinary and Life Sciences Faculty, University of Glasgow, G12 8QQ, UK.
Medical, Veterinary and Life Sciences Faculty, University of Glasgow, G12 8QQ, UK.
Prog Biophys Mol Biol. 2017 Jul;126:48-64. doi: 10.1016/j.pbiomolbio.2017.03.001. Epub 2017 Apr 25.
In Ca and Mg buffer solutions the ionised concentrations ([X]) are either calculated or measured. Calculated values vary by up to a factor of seven due to the following four problems: The calculated [X] in buffers are so inconsistent that calculation is not an option. Until standards are available, the [X] in the buffers must be measured. The Ligand Optimisation Method is an accurate and independently verified method of doing this (McGuigan & Stumpff, Anal. Biochem. 436, 29, 2013). Lack of standards means it is not possible to compare the published [Ca] in the nmolar range, and the apparent constant (K) values for Ca and Mg binding to intracellular ligands amongst different laboratories. Standardisation of Ca/Mg buffers is now essential. The parameters to achieve this are proposed.
在钙和镁缓冲溶液中，离子化浓度（[X]）通过计算或测量得出。由于以下四个问题，计算值的变化幅度高达七倍：缓冲液中计算出的[X]非常不一致，以至于无法进行计算。在有标准可用之前，必须测量缓冲液中的[X]。配体优化方法是一种准确且经过独立验证的方法（麦奎根和施图姆夫，《分析生物化学》436卷，第29页，2013年）。缺乏标准意味着无法比较纳摩尔范围内已发表的[钙]，以及不同实验室中钙和镁与细胞内配体结合的表观常数（K）值。钙/镁缓冲液的标准化现在至关重要。本文提出了实现这一目标的参数。
