Zhao Pengfei, Wang Shaochun, Zhou Yingjie, Zheng Huailiang, Zhao Gang
Luoyang Orthopedic-Traumatological Hospital, Luoyang, Henan 471002, P.R. China.
Exp Ther Med. 2017 Mar;13(3):1127-1132. doi: 10.3892/etm.2017.4052. Epub 2017 Jan 17.
The aims of the present study were to examine the expression of transforming growth factor (TGF)-β1 and microRNA (miR)-185 in the bone tissue, blood and cerebrospinal fluid of patients with spinal cord injuries and to evaluate the regulation of spinal cord injuries by miR-185. A total of 44 patients with spinal cord injuries induced by thoracolumbar spine compression fractures, who were hospitalized at Luoyang Orthopedic-Traumatological Hospital between June 2012 and February 2015 were enrolled in the present study. Among the patients enrolled, 18 underwent surgery between 1 and 7 days following fracture, and 26 patients underwent surgery between 8 and 14 days following fracture. Bone tissue, peripheral blood and cerebrospinal fluid were subsequently harvested from patients for analysis. Reverse transcription-quantitative polymerase chain reaction was performed to determine the expression of miR-185 and TGF-β1 mRNA. Western blotting was performed to evaluate TGF-β1 protein expression in bone tissue and ELISA was employed to quantify TGF-β1 protein expression in the blood and cerebrospinal fluid. TGF-β1 mRNA and protein levels in bone tissue, blood and cerebrospinal fluid from patients who underwent surgery 8-14 days post-fracture were significantly higher than those who underwent surgery 1-7 days post-fracture (P<0.05). By contrast, miR-185 levels were significantly lower in bone tissue, blood and cerebrospinal fluid from patients who underwent surgery 8-14 days post-fracture compared with those who underwent surgery 1-7 days post-fracture (P<0.05). The results of the present study desmonstrate that the upregulation of TGF-β1 in the bone tissue, blood and cerebrospinal fluid of patients with spinal cord injuries induced by thoracolumbar spine compression fractures is correlated with the downregulation of miR-185. Furthermore, miR-185 may target TGF-β1, affecting its transcription and translation, indicating that it serves an important role in spinal cord injuries induced by thoracolumbar spine compression fractures.
本研究的目的是检测脊髓损伤患者骨组织、血液和脑脊液中转化生长因子（TGF）-β1和微小RNA（miR）-185的表达，并评估miR-185对脊髓损伤的调控作用。本研究纳入了2012年6月至2015年2月期间在洛阳正骨医院住院的44例因胸腰椎压缩性骨折导致脊髓损伤的患者。在纳入的患者中，18例在骨折后1至7天接受手术，26例在骨折后8至14天接受手术。随后采集患者的骨组织、外周血和脑脊液进行分析。采用逆转录-定量聚合酶链反应检测miR-185和TGF-β1 mRNA的表达。采用蛋白质印迹法评估骨组织中TGF-β1蛋白的表达，采用酶联免疫吸附测定法量化血液和脑脊液中TGF-β1蛋白的表达。骨折后8至14天接受手术的患者骨组织、血液和脑脊液中TGF-β1 mRNA和蛋白水平显著高于骨折后1至7天接受手术的患者（P<0.05）。相比之下，骨折后8至14天接受手术的患者骨组织、血液和脑脊液中miR-185水平显著低于骨折后1至7天接受手术的患者（P<0.05）。本研究结果表明，胸腰椎压缩性骨折所致脊髓损伤患者骨组织、血液和脑脊液中TGF-β1的上调与miR-185的下调相关。此外，miR-185可能靶向TGF-β1，影响其转录和翻译，表明其在胸腰椎压缩性骨折所致脊髓损伤中起重要作用。
