Haidian Maternal & Child Health Hospital, Beijing, China; Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, China; Graduate School, Peking Union Medical College, Beijing, China.
FEBS Lett. 2014 Nov 28;588(23):4438-47. doi: 10.1016/j.febslet.2014.09.045.
MiR-185 expression has been associated with many cancers. However, the roles of miR-185 in human breast cancer remain elusive. Here, we found that miR-185 expression was decreased in human breast cancer tissues compared with healthy tissue controls. Up-regulation of miR-185 inhibited breast cancer cell proliferation and invasion and vice versa. MiR-185 was shown to bind to the 3′-untranslated region (UTR) of vascular endothelial growth factor a (Vegfa), and a significant inverse correlation was found between miR-185 and Vegfa. Vegfa overexpression partially restored the inhibition of cell proliferation and invasion that was induced by miR-185, and vice versa. Additionally, Vegfa expression was found to be high in human breast cancer tissues. Thus, miR-185-mediated Vegfa targeting may be involved in breast cancer formation.
miR-185 的表达与许多癌症有关。然而，miR-185 在人乳腺癌中的作用仍不清楚。在这里，我们发现与健康组织对照相比，miR-185 在人乳腺癌组织中的表达降低。miR-185 的上调抑制了乳腺癌细胞的增殖和侵袭，反之亦然。研究表明，miR-185 与血管内皮生长因子 a（Vegfa）的 3′-非翻译区（UTR）结合，并且在 miR-185 和 Vegfa 之间发现了显著的负相关关系。Vegfa 的过表达部分恢复了 miR-185 诱导的细胞增殖和侵袭的抑制，反之亦然。此外，在人乳腺癌组织中发现 Vegfa 的表达较高。因此，miR-185 介导的 Vegfa 靶向可能参与乳腺癌的形成。
