Hattori Hidemi, Ishihara Masayuki
Division of Biomedical Engineering, Research Institute, National Defense Medical College, Tokorozawa, Saitama 359-8513, Japan.
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Miyazaki 889-2192, Japan.
Exp Ther Med. 2017 Mar;13(3):1176-1180. doi: 10.3892/etm.2017.4041. Epub 2017 Jan 13.
Platelet-rich plasma (PRP) is blood plasma containing a high number of platelets that release growth factors for wound healing and tissue regeneration. In the present study, the feasibility of improving PRP therapy by using chitosan that exhibits high platelet activation ability was investigated. A total of 13 chitosan samples with different molecular weight (Mw) and degree of deacetylation (DDA) were individually added to blood samples of rats and the amount of growth factors, albumin and fibrinogen in plasma was measured. To examine the influence of plasma activated by chitosan on the proliferation of fibroblasts and adipose tissue-derived stromal cells (ASCs), the plasma was added to the culture medium of human fibroblasts and adipose tissue-derived stromal cells. Chitosan with a DDA of >75% increased the release of platelet factor 4 into the plasma. The amount of growth factors released into the plasma and platelet activation varied depending on the Mw and DDA, while albumin and fibrinogen were hardly affected. The proliferation rate was highest when using plasma activated by chitosan with a DDA of 75-85% and an Mw of 50,000-190,000 Da. These results suggested that the effectiveness of PRP therapy may be improved by using chitosan with a DDA of 75-85% and an Mw of 50,000-190,000 Da.
富含血小板血浆（PRP）是一种含有大量血小板的血浆，这些血小板可释放促进伤口愈合和组织再生的生长因子。在本研究中，研究了使用具有高血小板活化能力的壳聚糖来改善PRP疗法的可行性。将总共13种具有不同分子量（Mw）和脱乙酰度（DDA）的壳聚糖样品分别添加到大鼠血液样本中，并测量血浆中生长因子、白蛋白和纤维蛋白原的含量。为了检验壳聚糖激活的血浆对成纤维细胞和脂肪组织来源的基质细胞（ASC）增殖的影响，将该血浆添加到人类成纤维细胞和脂肪组织来源的基质细胞的培养基中。DDA>75%的壳聚糖增加了血小板因子4向血浆中的释放。释放到血浆中的生长因子量和血小板活化情况因Mw和DDA而异，而白蛋白和纤维蛋白原几乎不受影响。当使用DDA为75-85%且Mw为50,000-190,000 Da的壳聚糖激活的血浆时，增殖率最高。这些结果表明，使用DDA为75-85%且Mw为50,000-190,000 Da的壳聚糖可能会提高PRP疗法的有效性。
