Song Yanling, Tian Tian, Shi Yuanzhi, Liu Wenli, Zou Yuan, Khajvand Tahereh, Wang Sili, Zhu Zhi, Yang Chaoyong
MOE Key Laboratory of Spectrochemical Analysis & Instrumentation , The Key Laboratory of Chemical Biology of Fujian Province , State Key Laboratory of Physical Chemistry of Solid Surfaces , Collaborative Innovation Center of Chemistry for Energy Materials , Department of Chemical Engineering , Department of Chemical Biology , College of Chemistry and Chemical Engineering , Xiamen University , Xiamen 361005 , China . Email:
College of Biological Science and Engineering , Fuzhou University , Fuzhou 350116 , P. R. China.
Chem Sci. 2017 Mar 1;8(3):1736-1751. doi: 10.1039/c6sc04671a. Epub 2016 Dec 7.
Up to 90% of cancer-related deaths are caused by metastatic cancer. Circulating tumor cells (CTCs), a type of cancer cell that spreads through the blood after detaching from a solid tumor, are essential for the establishment of distant metastasis for a given cancer. As a new type of liquid biopsy, analysis of CTCs offers the possibility to avoid invasive tissue biopsy procedures with practical implications for diagnostics. The fundamental challenges of analyzing and profiling CTCs are the extremely low abundances of CTCs in the blood and the intrinsic heterogeneity of CTCs. Various technologies have been proposed for the enrichment and single-cell analysis of CTCs. This review aims to provide in-depth insights into CTC analysis, including various techniques for isolation of CTCs with capture methods based on physical and biochemical principles, and single-cell analysis of CTCs at the genomic, proteomic and phenotypic level, as well as current developmental trends and promising research directions.
高达90%的癌症相关死亡是由转移性癌症导致的。循环肿瘤细胞（CTCs）是一种从实体瘤脱离后通过血液扩散的癌细胞，对于特定癌症发生远处转移至关重要。作为一种新型的液体活检，对CTCs的分析提供了避免侵入性组织活检程序的可能性，对诊断具有实际意义。分析和描绘CTCs的根本挑战在于血液中CTCs的丰度极低以及CTCs的内在异质性。已经提出了各种技术用于CTCs的富集和单细胞分析。本综述旨在深入洞察CTCs分析，包括基于物理和生化原理的捕获方法分离CTCs的各种技术，以及在基因组、蛋白质组和表型水平对CTCs进行单细胞分析，以及当前的发展趋势和有前景的研究方向。
