Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada.
Department of Electrical and Computer Engineering, University of Toronto, Toronto, ON, Canada.
Angew Chem Int Ed Engl. 2016 Jan 22;55(4):1252-65. doi: 10.1002/anie.201505100. Epub 2015 Dec 8.
Over the last decade, significant progress has been made towards the development of approaches that enable the capture of rare circulating tumor cells (CTCs) from the blood of cancer patients, a critical capability for noninvasive tumor profiling. These advances have leveraged new insights in materials chemistry and microfluidics and allowed the capture and enumeration of CTCs with unprecedented sensitivity. However, it has become increasingly clear that simply capturing and counting tumor cells launched into the bloodstream may not provide the information needed to advance our understanding of the biology of these rare cells, or to allow us to better exploit them in medicine. A variety of advances have now emerged demonstrating that more information can be extracted from CTCs with next-generation devices and materials featuring tailored physical and chemical properties. In this Minireview, the last ten years of work in this area will be discussed, with an emphasis on the groundbreaking work of the last five years, during which the focus has moved beyond the simple capture of CTCs and gravitated towards approaches that enable in-depth analysis.
在过去的十年中，在开发能够从癌症患者的血液中捕获稀有循环肿瘤细胞（CTC）的方法方面取得了重大进展，这是一种非侵入性肿瘤分析的关键能力。这些进展利用了材料化学和微流控学的新见解，并允许以前所未有的灵敏度捕获和计数 CTC。然而，越来越明显的是，仅仅捕获和计数进入血液的肿瘤细胞可能无法提供推进我们对这些稀有细胞生物学理解所需的信息，也无法让我们在医学中更好地利用它们。现在已经出现了各种进展，表明具有定制物理和化学特性的下一代设备和材料可以从 CTC 中提取更多信息。在这篇综述中，将讨论该领域过去十年的工作重点是过去五年的开创性工作，在此期间，重点已从简单的 CTC 捕获转移到能够进行深入分析的方法上。
