Lee Maximillian T W, Maruani Antoine, Richards Daniel A, Baker James R, Caddick Stephen, Chudasama Vijay
Department of Chemistry , University College London , 20 Gordon Street , London , WC1H 0AJ , UK . Email:
Chem Sci. 2017 Mar 1;8(3):2056-2060. doi: 10.1039/c6sc03655d. Epub 2016 Nov 28.
The generation of antibody conjugates with a loading of two modules is desirable for a host of reasons. Whilst certain antibody engineering approaches have been useful in the preparation of such constructs, a reliable method based on a native antibody scaffold without the use of enzymes or harsh oxidative conditions has hitherto not been achieved. The use of native antibodies has several advantages in terms of cost, practicality, accessibility, time and overall efficiency. Herein we present a novel, reliable method of furnishing antibody conjugates with a loading of two modules starting from a native antibody scaffold.
出于诸多原因，生成带有两个模块负载量的抗体缀合物是很有必要的。虽然某些抗体工程方法在制备此类构建体方面很有用，但迄今为止尚未实现一种基于天然抗体支架且不使用酶或苛刻氧化条件的可靠方法。就成本、实用性、可及性、时间和整体效率而言，使用天然抗体具有若干优势。在此，我们提出了一种新颖、可靠的方法，可从天然抗体支架出发制备带有两个模块负载量的抗体缀合物。
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