Huff V, Cai W, Glorioso J C, Levine M
Department of Human Genetics, University of Michigan Medical School, Ann Arbor 48109-0618.
J Virol. 1988 Nov;62(11):4403-6. doi: 10.1128/JVI.62.11.4403-4406.1988.
Glycoprotein B (gB) is a virally encoded protein that is found in the envelope of herpes simplex virus type 1 and membranes of cells infected with herpes simplex virus type 1. It is essential for the production of infectious virus particles. An amber mutation was introduced into the gB gene by oligonucleotide-directed mutagenesis at the codon for amino acid 863 of the protein. Virus carrying this mutation should synthesize gB molecules lacking the last 41 amino acids of the cytoplasmic domain. Immunoprecipitation of infected cell extracts demonstrated the synthesis of appropriately truncated gB molecules. Characterization of the mutant virus indicated that the loss of the carboxy-terminal 41 amino acids has little effect on gB function.
糖蛋白B（gB）是一种病毒编码蛋白，存在于单纯疱疹病毒1型的包膜以及感染单纯疱疹病毒1型的细胞膜中。它对于传染性病毒颗粒的产生至关重要。通过寡核苷酸定向诱变，在该蛋白第863位氨基酸密码子处的gB基因中引入了琥珀突变。携带此突变的病毒应合成缺乏胞质结构域最后41个氨基酸的gB分子。感染细胞提取物的免疫沉淀显示合成了适当截短的gB分子。对突变病毒的特性分析表明，羧基末端41个氨基酸的缺失对gB功能影响不大。
