Faculty of Health, School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of TechnologyBrisbane, QLD, Australia.
Division of Neonatology, Cincinnati Children's Hospital Medical CentreCincinnati, OH, USA.
Front Cell Infect Microbiol. 2017 Apr 13;7:123. doi: 10.3389/fcimb.2017.00123. eCollection 2017.
The multiple banded antigen (MBA), a surface-exposed lipoprotein, is a proposed virulence factor of spp. We previously demonstrated that the number of MBA size variants in amniotic fluid was inversely proportional to the severity of chorioamnionitis in experimentally infected pregnant sheep. However, the effect of ureaplasma MBA size variation on inflammation in human pregnancies has not been reported. Ureaplasmas isolated from the chorioamnion of pregnant women from a previous study ( = 42) were speciated/serotyped and MBA size variation was demonstrated by PCR and western blot. Results were correlated with the severity of chorioamnionitis and cord blood cytokines. , THP-1-derived macrophages were exposed to recombinant-MBA proteins of differing sizes and NF-κB activation and cytokine responses were determined. MBA size variation was identified in 21/32 (65.6%) clinical isolates (in 10 clinical isolates MBA size variation was unable to be determined). Any size variation (increase/decrease) of the MBA (regardless of species or serovar) was associated with mild or absent chorioamnionitis ( = 0.023) and lower concentrations of cord blood cytokines IL-8 ( = 0.04) and G-CSF ( = 0.008). , recombinant-MBA variants elicited different cytokine responses and altered expression of NF-κB p65. This study demonstrates that size variation of the ureaplasma MBA protein modulates the host immune response and .
多带抗原（MBA）是一种表面暴露的脂蛋白，被认为是 spp 的毒力因子。我们之前的研究表明，羊感染模型中羊水 MBA 大小变异的数量与绒毛膜羊膜炎的严重程度呈反比。然而，脲原体 MBA 大小变化对人类妊娠炎症的影响尚未报道。我们从之前的研究中（n=42）分离了来自孕妇羊膜的脲原体，通过 PCR 和 Western blot 显示了 MBA 大小的变化。结果与绒毛膜羊膜炎的严重程度和脐血细胞因子相关。此外，我们还将 THP-1 衍生的巨噬细胞暴露于不同大小的重组 MBA 蛋白，测定 NF-κB 激活和细胞因子反应。在 21/32（65.6%）临床分离株中鉴定到 MBA 大小的变化（在 10 个临床分离株中，MBA 大小的变化无法确定）。MBA 的任何大小变化（增加/减少）（无论脲原体种属或血清型如何）都与轻度或无绒毛膜羊膜炎相关（p=0.023），脐血细胞因子 IL-8（p=0.04）和 G-CSF（p=0.008）的浓度降低相关。此外，重组 MBA 变体引起不同的细胞因子反应，并改变 NF-κB p65 的表达。本研究表明，脲原体 MBA 蛋白的大小变化调节宿主免疫反应。
