Ali I, Zhang S, Muhammad M S, Iqbal M, Cui J-J J-J
State Key Lab of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.
Dept of Entomology, Univ College of Agriculture and Environmental Sciences, The Islamia Univ of Bahawalpur, Baghdad ul-jadeed Campus, Bahawalpur, 63100, Pakistan.
Neotrop Entomol. 2018 Jun;47(3):336-343. doi: 10.1007/s13744-017-0526-y. Epub 2017 Apr 28.
Biosafety of a genetically modified crop is required to be assessed prior to its commercialization. For this, a suitable artificial diet was developed and used to establish a dietary exposure test for assessing the toxicity of midgut-active Bt insecticidal proteins on Chrysopa pallens (Rambur). Subsequently, this dietary exposure test was used to evaluate the toxicity of the proteins Cry1Ab, Cry1Ac, Cry1Ah, Cry1Ca, Cry1F, Cry2Aa, Cry2Ab, and Vip3Aa on C. pallens larvae. Temporal stability, bioactivity, and the intake of the insecticidal proteins were confirmed by enzyme-linked immunosorbent assay and a sensitive-insect bioassay. The life history characteristics, such as survival, pupation, adult emergence, 7-day larval weight, larval developmental time, and emerged male and female fresh weights remained unaffected, when C. pallens were fed the pure artificial diet (negative control) and the artificial diets containing 200 μg/g of each purified protein: Cry1Ab, Cry1Ac, Cry1Ah, Cry1Ca, Cry1F, Cry2Aa, Cry2Ab, or Vip3Aa. On the contrary, all of the life history characteristics of C. pallens larvae were adversely affected when fed artificial diet containing boric acid (positive control). The results demonstrate that diets containing the tested concentrations of Cry1Ab, Cry1Ac, Cry1Ah, Cry1Ca, Cry1F, Cry2Aa, Cry2Ab, and Vip3Aa have null effects on C. pallens larvae. The outcome indicates that genetically modified crops expressing the tested Bt proteins are safe for the lacewing, C. pallens.
转基因作物在商业化之前需要对其生物安全性进行评估。为此，开发了一种合适的人工饲料，并用于建立饮食暴露试验，以评估中肠活性Bt杀虫蛋白对中华草蛉（Rambur）的毒性。随后，该饮食暴露试验被用于评估Cry1Ab、Cry1Ac、Cry1Ah、Cry1Ca、Cry1F、Cry2Aa、Cry2Ab和Vip3Aa蛋白对中华草蛉幼虫的毒性。通过酶联免疫吸附测定和敏感昆虫生物测定法确认了杀虫蛋白的时间稳定性、生物活性和摄入量。当用纯人工饲料（阴性对照）和含有200μg/g每种纯化蛋白（Cry1Ab、Cry1Ac、Cry1Ah、Cry1Ca、Cry1F、Cry2Aa、Cry2Ab或Vip3Aa）的人工饲料喂养中华草蛉时，其生存、化蛹、羽化、7日龄幼虫体重、幼虫发育时间以及羽化后的雌雄鲜重等生活史特征均未受到影响。相反，当用含硼酸的人工饲料（阳性对照）喂养中华草蛉幼虫时，其所有生活史特征均受到不利影响。结果表明，含有测试浓度的Cry1Ab、Cry1Ac、Cry1Ah、Cry1Ca、Cry1F、Cry2Aa、Cry2Ab和Vip3Aa的饲料对中华草蛉幼虫无影响。结果表明，表达测试Bt蛋白的转基因作物对草蛉中华草蛉是安全的。
