Pinto Matheus Moreira Dantas, Mishra Swati, De Bortoli Sergio Antonio, Jurat-Fuentes Juan Luis
Department of Entomology and Plant Pathology, University of Tennessee, 370 E. J. Chapman Dr, Knoxville, TN 37996, USA.
Department of Plant Protection, School of Agricultural and Veterinarian Sciences, São Paulo State University, Access Prof. Paulo Donato Castellane s/n, Jaboticabal, São Paulo 14884-900, Brazil.
J Insect Sci. 2025 Jul 15;25(4). doi: 10.1093/jisesa/ieaf058.
The Colorado potato beetle (CPB, Leptinotarsa decemlineata (Say)) is the most important defoliator of solanaceous crops. Control of this pest is hindered by its ability to develop resistance to insecticides, including insecticidal proteins from Bacillus thuringiensis (Bt) Berliner. Therefore, it is important to find alternative tools that may be combined into an integrated pest management approach for CPB control. In this study, we evaluated the efficiency of the combined use of the Cry3Aa protein from Bt and Chrysoperla rufilabris (Burmeister) in controlling CPB. Control of CPB larval second instar at different densities by 3 larval instars of the predator was tested in potato leaves treated with an LC50 concentration of the Cry3Aa protein. Potato leaf damage was evaluated in experiments where CPB larvae were fed with leaves treated with Cry3Aa protein and exposed to predator larvae for 7 days or until all CPB larvae were dead. Results show that all 3 instars of the predator presented a type II functional response for all conditions evaluated. The first and second larval instars of C. rufilabris larvae attacked twice the number of prey (1.34-2.91 and 2.9-4.75, respectively) when CPB larvae were fed on leaves with the Cry3Aa protein. A complete reduction in the number of CPB larvae and lower levels of potato leaf damage was observed when the predator larvae were present. These results support the combined use of the Cry3Aa protein with C. rufilabris in increasing the efficiency of CPB control.
科罗拉多马铃薯甲虫（CPB，Leptinotarsa decemlineata (Say)）是茄科作物最重要的食叶害虫。这种害虫对杀虫剂产生抗性的能力，包括对苏云金芽孢杆菌（Bt）Berliner的杀虫蛋白产生抗性，阻碍了对其的防治。因此，找到可纳入综合虫害管理方法以控制CPB的替代工具很重要。在本研究中，我们评估了Bt的Cry3Aa蛋白与红带食蚜蝇（Chrysoperla rufilabris (Burmeister)）联合使用对控制CPB的效果。在用Cry3Aa蛋白的LC50浓度处理过的马铃薯叶片上，测试了捕食者的3个幼虫龄期对不同密度CPB二龄幼虫的控制效果。在实验中，让CPB幼虫取食经Cry3Aa蛋白处理的叶片，并使其接触捕食者幼虫7天或直至所有CPB幼虫死亡，然后评估马铃薯叶片的损伤情况。结果表明，在所评估的所有条件下，捕食者的所有3个龄期均呈现出II型功能反应。当CPB幼虫取食含有Cry3Aa蛋白的叶片时，红带食蚜蝇幼虫的一龄和二龄幼虫攻击的猎物数量是原来的两倍（分别为1.34 - 2.91和2.9 - 4.75）。当存在捕食者幼虫时，观察到CPB幼虫数量完全减少，且马铃薯叶片损伤程度降低。这些结果支持将Cry3Aa蛋白与红带食蚜蝇联合使用，以提高控制CPB的效率。
