Lo Vasco Vincenza Rita, Leopizzi Martina, Di Maio Valeria, Di Raimo Tania, Cesa Stefania, Masci Alessandra, Rocca Carlo Della
Department of Sensory Organs, Faculty of Medicine and Dentistry, Policlinico Umberto I, Sapienza University of Rome, viale dell'Università 33, 00161, Rome, Italy.
Department of Medico-Surgical Sciences and Biotechnology, Polo Pontino, Sapienza University, Latina, Rome, Italy.
J Cell Commun Signal. 2017 Dec;11(4):317-327. doi: 10.1007/s12079-017-0391-9. Epub 2017 Apr 27.
Endothelial cells (EC) act as leading actors in angiogenesis. Understanding the complex network of signal transduction pathways which regulate angiogenesis might offer insights in the regulation of normal and pathological events, including tumours, vascular, inflammatory and immune diseases. The effects of olive oil and of Blueberry extracts upon the phosphoinositide (PI)-specific phospholipase C (PLC) enzymes were evaluated both in quiescent and inflammatory stimulated human umbilical vein EC (HUVEC) using molecular biology (multiliquid bioanalysis) and immunofluorescence techniques. Oleuropein significantly increased the number of surviving HUVEC compared to untreated controls, suggesting that it favours the survival and proliferation of EC. Our results suggest that Oleuropein might be useful to induce EC proliferation, an important event during angiogenesis, with special regard to wound healing. Blueberry extracts increased the number of surviving HUVEC, although the comparison to untreated controls did not result statistically significant. Lipopolysaccharide (LPS) administration significantly reduced the number of live HUVEC. LPS can also modify the expression of selected PLC genes. Adding Blueberry extracts to LPS treated HUVEC cultures did not significantly modify the variations of PLC expression induced by LPS. Oleuropein increased or reduced the expression of PLC genes, and statistically significant results were identified for selected PLC isoforms. Oleuropein also modified the effects of LPS upon PLC genes' expression. Thus, our results corroborate the hypothesis that Oleuropein owns anti-inflammatory activity. The intracellular localization of PLC enzymes was modified by the different treatments we used. Podosome-like structures were observed in differently LPS treated HUVEC.
内皮细胞（EC）在血管生成过程中起主要作用。了解调节血管生成的复杂信号转导通路网络，可能为包括肿瘤、血管、炎症和免疫疾病在内的正常和病理事件的调控提供见解。利用分子生物学（多液体生物分析）和免疫荧光技术，评估了橄榄油和蓝莓提取物对静止和炎症刺激的人脐静脉内皮细胞（HUVEC）中磷酸肌醇（PI）特异性磷脂酶C（PLC）酶的影响。与未处理的对照相比，橄榄苦苷显著增加了存活的HUVEC数量，表明它有利于内皮细胞的存活和增殖。我们的结果表明，橄榄苦苷可能有助于诱导内皮细胞增殖，这是血管生成过程中的一个重要事件，尤其对于伤口愈合而言。蓝莓提取物增加了存活的HUVEC数量，尽管与未处理的对照相比，差异无统计学意义。脂多糖（LPS）处理显著减少了存活的HUVEC数量。LPS还可改变所选PLC基因的表达。向LPS处理的HUVEC培养物中添加蓝莓提取物，并未显著改变LPS诱导的PLC表达变化。橄榄苦苷增加或减少了PLC基因的表达，并且所选PLC同工型的结果具有统计学意义。橄榄苦苷还改变了LPS对PLC基因表达的影响。因此，我们的结果证实了橄榄苦苷具有抗炎活性这一假设。我们使用的不同处理改变了PLC酶的细胞内定位。在不同LPS处理的HUVEC中观察到了类似小体的结构。
