Suffredini Elisabetta, Proroga Yolande Thérèse Rose, Di Pasquale Simona, Di Maro Orlandina, Losardo Maria, Cozzi Loredana, Capuano Federico, De Medici Dario
Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy.
Department of Food Inspection, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute 2, Portici, 80055, Naples, Italy.
Food Environ Virol. 2017 Dec;9(4):423-433. doi: 10.1007/s12560-017-9302-8. Epub 2017 Apr 27.
The aim of this study was to assess the trend of hepatitis A virus (HAV) in a coastal zone impacted by a contamination event, providing data for the development of management strategies. A total of 352 samples, including four bivalve mollusc species (Mytilus galloprovincialis, Solen vagina, Venus gallina and Donax trunculus), were taken over a period of 6 months from 27 production areas of the coast and analysis were performed according to ISO/TS 15216-1:2013. HAV presence was detected in 77 samples from 11 production areas and all positive results were related to samples collected in the first 3 months of the surveillance, during which HAV prevalence was 39.9% and values as high as 5096 genome copies/g were detected. A progressive reduction of viral contamination was evident during the first trimester of the monitoring, with prevalence decreasing from 78.8% in the first month, to 37.8% in the second and 3.9% in the third and quantitative levels reduced from an average value of 672 genome copies/g to 255 genome copies/g over a period of 4 weeks (virus half-life: 21.5 days). A regression analysis showed that, during the decreasing phase of the contamination, the data fitted a reciprocal quadratic model (Ra = 0.921) and, based on the model, a residual presence of HAV could be estimated after negativization of the production areas. The statistical analysis of the results per shellfish species and per production area showed that there were limited differences in contamination prevalence and levels among diverse bivalve species, while a statistically significant difference was present in quantitative levels of one production area. These data could be useful for the development of both risk assessment models and code of practice for the management of viral contamination in primary production.
本研究的目的是评估受污染事件影响的沿海地区甲型肝炎病毒（HAV）的趋势，为制定管理策略提供数据。在6个月的时间里，从沿海27个产区采集了总共352个样本，包括四种双壳贝类（地中海贻贝、缢蛏、紫贻贝和紫彩血蛤），并按照ISO/TS 15216-1:2013进行分析。在11个产区的77个样本中检测到了HAV的存在，所有阳性结果均与监测前3个月采集的样本有关，在此期间HAV患病率为39.9%，检测到的值高达5096基因组拷贝/克。在监测的前三个月中，病毒污染明显逐渐减少，患病率从第一个月的78.8%降至第二个月的37.8%，第三个月降至3.9%，定量水平在4周内从平均672基因组拷贝/克降至255基因组拷贝/克（病毒半衰期：21.5天）。回归分析表明，在污染减少阶段，数据符合倒数二次模型（Ra = 0.921），根据该模型，可以估计产区阴性化后HAV的残留存在情况。对每个贝类物种和每个产区的结果进行统计分析表明，不同双壳贝类物种之间的污染患病率和水平差异有限，而一个产区的定量水平存在统计学上的显著差异。这些数据对于开发风险评估模型和初级生产中病毒污染管理的操作规范可能是有用的。
