Bigoraj Ewelina, Kwit Ewa, Chrobocińska Marta, Rzeżutka Artur
Department of Food and Environmental Virology, National Veterinary Research Institute, Al. Partyzantów 57, 24-100, Puławy, Poland.
Food Environ Virol. 2014 Sep;6(3):207-12. doi: 10.1007/s12560-014-9153-5. Epub 2014 Jun 7.
The aim of the study was to define the occurrence of human noroviruses of genogroup I and II (NoV GI and NoV GII) and hepatitis A virus (HAV) in the Baltic Sea mussels. The shellfish samples were taken at the sampling sites located on the Polish coast. In total, 120 shellfish were tested as pooled samples using RT-PCR and hybridisation with virus specific probes. NoV GI was detected in 22 (18.3%), NoV GII in 28 (23.3%), and HAV in 9 (7.5%) of the shellfish. The nucleotide sequence analysis of the detected NoV GII strains showed a 97.3-99.3% similarity to GII.4 virus strain. This is the first report describing the NoV and HAV occurrence in wild Baltic mussels and their possible role as bioindicators of seawater contamination with human enteric viruses.
该研究的目的是确定波罗的海贻贝中基因I群和II群人诺如病毒（NoV GI和NoV GII）以及甲型肝炎病毒（HAV）的存在情况。贝类样本取自波兰海岸的采样点。总共120份贝类样本作为混合样本使用逆转录聚合酶链反应（RT-PCR）和病毒特异性探针杂交进行检测。在22份（18.3%）贝类样本中检测到NoV GI，28份（23.3%）中检测到NoV GII，9份（7.5%）中检测到HAV。对检测到的NoV GII毒株进行的核苷酸序列分析显示，其与GII.4病毒毒株的相似度为97.3%-99.3%。这是第一份描述野生波罗的海贻贝中NoV和HAV的存在情况以及它们作为人类肠道病毒海水污染生物指示物可能作用的报告。
